酯基型Gemini双季铵盐表面活性剂与DNA的相互作用  

Interaction of DNA and gemini quaternary ammonium salt surfactants with eater group

作　　者：延秀银 王渊[2] 常宏宏[2,3] YAN Xiu-yin WANG YUAN CHANG Hong-hong(Taiyuan Control Center of Grain, Taiyuan 030013 College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024 Opening Funding Supported by the Key Laboratory of Road Structure＆Material of Transport, Beijing 100088)

机构地区：[1]太原市粮食质量监督检测站,太原030013 [2]太原理工大学化学化工学院,太原030024 [3]道路结构与材料交通运输行业重点实验室(北京),北京100088

出　　处：《分析试验室》2017年第10期1136-1140,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(21206103;21076135);道路结构与材料交通运输行业重点实验室开放基金(KF201403);山西省科技攻关项目(20140321018-05);山西省自然科学基金(2015011023);表面活性剂山西省重点实验室开放基金(201503)资助

摘　　要：在合成系列酯基型Gemini双季铵盐表面活性剂的基础上,采用紫外可见分光光度法和荧光分光光度法考察了系列表面活性剂与DNA的相互作用。酯基型Gemini双季铵表面活性剂均未使DNA的吸收峰发生红移,即两者相互作用时没有发生嵌插作用,且与DNA相互作用的猝灭属于静态猝灭。当连接基相同时,结合常数随疏水链的增长呈先增大后减小的趋势,但当疏水链长度相同时,连接基碳链长度的变化对结合常数的影响并无明显规律。This article designed synthesize Gemini quaternary ammonium salt surfactants with eater group, the interaction between Gemini surfaetant and DNA were investigated by methods of using UV- vis and fluorometric spectrophotometry. The absorption peak of DNA was not red shifted when Gemini surfactants were added, but suefactants had hyperchromic effect to DNA, the binding constant of static quenching theory model indicated that, while the binding constant between Gemini surfactants with ester group is bigger when the length of hydrophobic chains is longer. As for Gemini surfactants with ester group, when length of hydrophobic chains is shorter, the binding constant increase with the longth of spacer group, while length of hydrophobic chains became longer, the contrary happens.

关 键 词：GEMINI表面活性剂 DNA 酯基 吸收光谱 相互作用 

分 类 号：TQ041.7[化学工程]