鸟嘌呤在铂纳米/壳聚糖/石墨烯修饰玻碳电极上的电化学行为及测定  

Electrochemical behaviors of guanine at the Pt NGs/CS/RGO/GCE and its determination

作　　者：张娜 张克营 王红艳 徐基贵 史洪伟 王聪 耿涛 孙洁 ZHANG Na ZHANG Ke-ying WANG Hong-yan XU Ji-gui SHI Hong-wei WANG Cong GENG Tao SUN Jie(School of Chemistry and Chemical Engineering, Suzhou Universit Anhui Key Laboratory of Spin Electron and Nanomaterials, Suzhou 234000)

机构地区：[1]安徽省宿州学院化学化工学院自旋电子与纳米材料安徽省重点实验室,宿州234000

出　　处：《分析试验室》2017年第10期1170-1172,共3页Chinese Journal of Analysis Laboratory

基　　金：宿州学院科研平台开放课题(2015ykf16;2011YKF03);宿州学院大学生科研立项(KYLXLKYB16-04;KYLXLKZD15-01;KYLXLKYB15-25);宿州学院优秀青年人支支持计划资助项目(SZXYQNL7017001)资助;安徽省教育厅自然科学基金重点项目(KJ2017A434;KJ2016A888);国家创新创业项目(201510379037);省级创新创业项目(201510379051)

摘　　要：制备了铂纳米/壳聚糖/石墨烯修饰电极。研究了鸟嘌呤(G)在修饰电极上的电化学行为。结果表明,该修饰电极对G的氧化具有明显的电催化作用。利用示差脉冲伏安法(DPV)对G进行检测,在0.1~10μmol/L浓度范围内,G的氧化峰电流和浓度成良好的线性关系,线性方程为I(μA)=2.08-1.92c(×10^(-5)mol/L),相关系数为0.9977,检测限为32 nmol/L(S/N=3)。该修饰电极用于血清样品中G的检测。PtNGs/CS/RGO/GCE was fabricated. The electrochemical behaviors of guanine at the modified electrode were investigated. Experimental results indicated that the electrode showed a good electrocatalytic ability for the oxidation of guanine. DPV was used to detect guanine. The oxidation peak current of guanine was good linear with its concentration at the range of 0. 1 - 10 μmol/L. The linear regression equation was I（p,A） = 2.08 - 1.92c （10-Smol/L） （r = 0. 9977） with the detection limit of 32 nmol/L （S/N = 3 ）. In addition, the electrode exhibited favorable good stability, reproducibility and anti-interference. It was used to detect guanine in serum sample with satisfactory results

关 键 词：石墨烯 鸟嘌呤 铂纳米 壳聚糖 

分 类 号：Q503[生物学—生物化学]