纳米金标记共振光散射法均相测定神经元特异性烯醇化酶  

Homogeneous resonance light scattering spectral assay of neuron specific enolase based on gold labeling

作　　者：邹明静[1] 王淑玉[2] 王英玲[1] ZOU Ming-jing WANG Shu-yu WANG Ying-ling(Central Laboratory, Heze Medical College, Heze 274030 Clinical Laboratory, Heze City Hospital, Heze 274000)

机构地区：[1]菏泽医学专科学校中心实验室,菏泽274030 [2]山东菏泽市立医院检验科,菏泽274000

出　　处：《分析试验室》2017年第10期1198-1201,共4页Chinese Journal of Analysis Laboratory

基　　金：山东省高等学校科技计划项目(J11LB01)资助

摘　　要：采用纳米金(15 nm)标记神经元特异性烯醇化酶抗体(anti-NSE)获得金标记anti-NSE。在适宜的条件下,金标记anti-NSE可与神经元特异性烯醇化酶(NSE)发生免疫反应生成NSE的免疫金复合物,使纳米金发生凝集,该液相体系在620 nm处共振光散射信号显著增强。实验表明,NSE质量浓度在0.05~20 ng/m L范围内与共振光散射增强值呈现良好的线性关系,检出限为0.01 ng/m L,方法已用于人血清中NSE的定量分析。Gold nanoparticles of 15 nm were attached to neuron specific enolase （anti-NSE）. The immune reaction between neuron specific enolase （NSE） and gold-labeled anti-NSE occurred in certain condition, and the resonance light scattering intensity at 620 nm increased significantly. △I620nm had a good linear relationship with NSE concentrations in range of O. 05 -20 ng/mL, getting the detection limit of 0.01 ng/mL NSE. It was used for the quantitative detecting of NSE in the clinical diagnosis with good results.

关 键 词：纳米金 神经元特异性烯醇化酶 金标记 共振光散射 

分 类 号：Q503[生物学—生物化学]