荧光增强型水合肼香豆素荧光探针的合成与应用  

Synthesis and application of coumarin fluorescence enhancement probe for N_2H_4 detection

作　　者：侯鹏[1] 董玉晶[1] 李爽[1] 许凤[1] HOU Peng DONG Yu-fing LI Shuang XU Feng(College of Pharmacy, Qiqihar Medical University, Qiqihar 161006)

机构地区：[1]齐齐哈尔医学院药学院,齐齐哈尔161006

出　　处：《分析试验室》2017年第10期1206-1209,共4页Chinese Journal of Analysis Laboratory

基　　金：齐齐哈尔市科学技术计划项目(SFGG-201546)资助

摘　　要：基于水合肼诱导乙酰基脱保护生成亚胺香豆素的原理,设计合成了一种荧光增强型检测水合肼的探针。相对于其他测试物(Cl^-,ClO_4^-,AcO^-,CO_3^(2-),H_2PO_4^-,K^+,Mg^(2+),Ca^(2+),Fe^(2+),Fe^(3+),Cys,GSH,NH_2OH,尿素,苯胺,硫脲,氨水,乙二胺),目标探针表现出对N2H4较高的选择性和专一性,探针溶液在500 nm处呈现显著的荧光恢复(25倍),探针溶液的荧光恢复与加入的水合肼浓度成正向关系,拟合方程为y=17.4309+4.5968x(R=0.9992),检出限2.7×10^(-8)mol/L。细胞荧光成像实验证明了合成的目标探针具有检测生物体系内水合肼的能力。The mechanism of action was based on the N2H4-triggered cleavage of the acetyl moiety to give iminocoumarin, and a highly sensitivity, efficient and good selectivity fluorescence enhancement probe for detecting hydrazine was synthesized. Compared with other analytes （ Cl-, ClO4-, AcO-, CO32-, H2PO4-, K＋, Mg2＋, Ca2＋, Fe2＋, Fe3＋, Cys, GSH, NH2OH, aniline, urea, thiourea, ammonia and ethylenediamine）, the probe showed a high selectivity and specificity for N2 Ha. The fluorescence spectra of probe solution showed a significant fluorescence recovery （25 fold ）. The fluorescence recovery of the probe solution was positively correlated with the concentration of hydrazine. The fitting equation was y = 17. 4309 ＋4. 5968x （R =0. 9992）, and the detection limit was 2.7 × 10-8 mol/L. Meanwhile, intracellular fluorescence imaging experiments showed that the fluorescence probe had the potential ability for N2H4 detection in vivo.

关 键 词：水合肼 香豆素 荧光增强 合成 

分 类 号：Q503[生物学—生物化学]