SUMO-1在ApoE-/-小鼠PM_(2.5)暴露中调控血管HIF-1α/VEGF信号通路的研究  

HIF-1α/VEGF Signal Pathway Regulated by SUMO-1 in Vessel after ApoE-/-Mice Exposed to PM_(2.5)

作　　者：甘向东 李飞[1] 蔡欣[1] 付文亮[1] 徐东刚[1] 龙民慧[2] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850 [2]天津科技大学生物工程学院,天津300457

出　　处：《华南师范大学学报（自然科学版）》2017年第5期59-63,共5页Journal of South China Normal University(Natural Science Edition)

基　　金：国家重点基础研究计划(973)项目(2011CB503803);国家自然科学基金项目(81170255;31271476)

摘　　要：以气管滴注方法研究了Apo E-/-小鼠经PM_(2.5)暴露后,其主动脉弓中SUMO-1、HIF-1α、血管内皮生长因子(VEGF)表达变化规律及HIF-1α的SUMO化修饰情况.实验结果显示:PM_(2.5)暴露后,小鼠主动脉SUMO-1、HIF-1α、VEGF的表达上调;PM_(2.5)暴露诱导了SUMO-1对HIF-1α的SUMO化修饰;低氧条件下SUMO-1敲低导致HIF-1α表达的下调.结果提示PM_(2.5)暴露通过上调细胞中SUMO-1的表达,稳定或者上调HIF-1α的表达,进而影响血管内皮生长因子(VEGF)的表达,造成动脉粥样硬化斑块的不稳定.To evaluated the regulation of SUMO-1 to HIF-1α and vascular endothelial growth factor(VEGF) in Apo E-/-mice after PM_(2.5) exposure by intratracheal instillation method,the SUMOylation of HIF-1α by SUMO-1,the expressions of SUMO-1,HIF-1α,and VEGF were investigated. After PM_(2.5) exposure,the expressions of SUMO-1,HIF-1α,and VEGF in mouse aorta were up-regulated remarkably. PM_(2.5) exposure induced SUMO-1 mediated SUMOlytion of HIF-1α. The expression of HIF-1α was down-regulated by SUMO-1 knockdown under hypoxia. These results suggest that PM_(2.5) exposure up-regulates the expression of SUMO-1 and stabilizes or up-regulates expression of HIF-1α,then upregulates the expression of VEGF,causing the unstablility of atherosclerotic plaques.

关 键 词：PM2.5暴露 SUMO-1 缺氧诱导因子-1Α 血管内皮生长因子 

分 类 号：R318[医药卫生—生物医学工程]