钛合金表面聚合氨基阳离子纳米薄膜的制备及抗菌性能的初步研究  

Fabrication and antibacterial property of amino cationic nanofilm on surface of titanium alloy :a preliminary study

作　　者：陈睿[1] 何海龙[1] 袁文[1] 田野[1] 秦余磊 赵鹏[2] 董敏杰 

机构地区：[1]第二军医大学附属长征医院脊柱外科,上海200003 [2]同济大学纳米研究所,上海200092

出　　处：《脊柱外科杂志》2017年第5期299-303,共5页Journal of Spinal Surgery

基　　金：上海市科委纳米专项课题(12nm0501202)

摘　　要：目的制备钛合金表面聚合氨基阳离子纳米薄膜并初步评价其抗菌性能。方法通过低温等离子体聚合技术(Plasma)使烯丙胺单体于钛合金材料表面引发聚合,制备带表面涂层的钛合金样品。采用扫描电子显微镜(SEM)观察样品表面形貌,水接触角测量仪分析表面亲水性,X线光电子能谱仪(XPS)分析表面元素含量,体外抗菌实验测试样品对金黄色葡萄球菌及大肠杆菌的抑菌率。在新西兰大白兔胫骨的骨髓腔内注入金黄色葡萄球菌,然后置入带涂层的钛合金螺钉,观察体内抗菌实验效果。结果采用Plasma方法制备的带涂层的钛合金样品表面散在颗粒样聚合物,水接触角由不带涂层的对照组的100.3°±3.4°下降至13.6°±3.0°,差异具有统计学意义(P<0.05);X线光电子能谱仪显示氮(N)元素含量由对照组的2.75%提高至11.80%。体外抗菌实验中,与对照组相比,涂层组对金黄色葡萄球菌的抑菌率为(86.1±3.8)%,对大肠杆菌的抑菌率为(75.5±2.6)%,差异具有统计学意义(P<0.05)。动物实验中,与对照组相比,涂层组螺钉周围中性粒细胞明显减少。结论低温等离子体聚合技术可以在钛合金表面成功制备聚合氨基阳离子纳米薄膜,制品具有良好的抗菌性,为聚合阳离子进一步的抗菌研究提供基础。Objective To fabricate amino cationic polymer on the surface of titanium alloys and evaluate its antibacterial property. Methods Amine cationic polymer was deposited on the surface of titanium rod with plasma polymerization technique by introducing allyl amine. The surface topography and wettability of the samples were observed with scanning electron microscope（SEM） and contact angle. Surface element contents were analyzed by X-ray photoelectron spectroscopy（XPS）. The antibacterial activity was measured by bacteria counting of Staphylococcus aureus and Escherichia coli in vitro. Antibacterial experiment in vivo in rabbit was conducted and histological analysis performed. Results Diffused distribution of spherical particles was observed under SEM on the surface. Water contact angle reduced from 100.3°±3.4° to 13.6°±3.0°,which was of a statistical significance（P〈0.05）. XPS results showed the content of N element increased from 2.75% to 11.8%. Bacteriostasis rate of Staphylococcus aureus was（86.1±3.8）% and that of Escherichia coli was（75.5±2.6）% in plasma group,which has a statistical significance in difference copmpared with the control group（P〈0.05）. In vivo,histological analysis demonstrated that decrease of neutrophils was observed around the implant and bone in plasma group. Conclusion Plasma polymerization technique can acquire amino cationic polymer on titanium surface successfully,and the polymer shows good antibacterial activity,thus providing the basis for future experiments.

关 键 词：钛 假体和植入物 细菌感染 纳米医学 

分 类 号：R318.08[医药卫生—生物医学工程]