丹参通络解毒汤联合骨髓干细胞动员对心肌缺血再灌注损伤大鼠内皮的保护作用  

Protective Effect of Danshen Tongluo Jiedu Decoction Combined with Bone Marrow Stem Cells Mobilization on the Vascular Endothelial Cells in Rats with Myocardial Ischemia Reperfusion Injury

作　　者：李鑫辉 黄淼鑫 杜建芳 黄政德 许福丽 肖青 郭晨鹤 李彩云 

机构地区：[1]湖南中医药大学中医学院,湖南长沙410208

出　　处：《湖南中医药大学学报》2017年第10期1070-1073,共4页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金面上项目(30973750);湖南省教育厅科学研究研重点项目(15A143);湖南省中医药科研计划项目(201559);湖南中医药大学研究生创新课题(2016cx21)

摘　　要：目的通过观察心肌缺血再灌注损伤大鼠NO水平、eNOS蛋白和eNOS mRNA表达,探讨丹参通络解毒汤联合骨髓干细胞动员对心肌缺血再灌注损伤大鼠内皮保护作用。方法大鼠60只,随机分为假手术组、模型组、重组人红细胞生成素(recombinant human erthropoietin,rh EPO)动员组、丹参通络解毒汤组;丹参通络解毒汤联合动员组,每组12只。于造模后14 d采用放射免疫法测定NO水平、免疫组化法测定eNOS蛋白和采用实时荧光定量PCR法测定eNOS mRNA表达。结果与假手术组比较,各组NO水平,eNOS蛋白和eNOS mRNA表达均明显降低,差异具有统计学意义(P<0.01);与模型组比较,rh EPO动员组、丹参通络解毒汤组和丹参通络解毒汤联合动员组NO水平升高,eNOS蛋白和eNOS mRNA表达明显提高,差异具有统计学意义(P<0.01);与rh EPO动员组和丹参通络解毒汤组比较,丹参通络解毒汤联合动员组NO水平显著升高,eNOS蛋白和eNOS mRNA表达明显提高,差异具有统计学意义(P<0.01)。结论丹参通络解毒汤可以提高缺血心肌eNOS mRNA表达,进一步促进eNOS蛋白表达,升高NO水平,是该方保护心肌缺血再灌注损伤大鼠内皮重要机制之一,同时该方联合动员剂对内皮保护有增强促进作用。Objective To investigate the protective effect of Danshen Tongluo Jiedu decoction combined with bone marrow stem cells mobilization on vascular endothelial cells through observing the changes of NO level, eNOS protein and mRNA eNOS expression in rats with myocardial ischemia reperfusion injury Methods The 60 rats were randomly divided into sham-operation group, model group, rh EPO mobilization group, Danshen Tongluo Jiedu decoction（DTJD） group, and Dan Shen Tong Luo Jie Du decoction combined with rh EPO mobilization（DTJr） group, 12 rats in each group. After 14 days of modeling, the NO level was determiened by radioimmunaossay. The expression of eNOS protein was assessed by immunocytochemistry method, and eNOS mRNA was measured by real-time fluorescence quantitative PCR method. Results Compared with sham-operation group,the levels of NO, eNOS and eNOS reduced significantly（P〈0.01）. Compared with the IRI group, the levels of NO, eNOS protein and mRNA eNOS expression in rh EPO mobilization group, DTJD group and DTJDr group increased significantly, the differences were statistically significant（P〈0.01）. Cmpared with rh EPO mobilization group and DTGD group, the level of NO, eNOS protein and mRNA eNOS expression in DTJr group increased significantly, the differences were statistically significant（P〈0.01） Conclusion DTJD could improve the expression of eNOS mRNA in endothelial cells and further promote expression of eNOS protein. It is may be one of the mechanisms of protecting endothelial cells in rats with myocardial ischemia reperfusion injury. DTJD combined with mobilization agent could enhance the protective effect on endothelial cells.

关 键 词：ENOS 心肌缺血再灌注损伤 骨髓干细胞 丹参通络解毒汤 

分 类 号：R285.5[医药卫生—中药学] R541.4[医药卫生—中医学]