组合浸矿细菌菌群的氧化特性及其群落结构分析  

Oxidation Characteristics and Community Structure of a Mixed Bioleaching Bacterial Consortium

作　　者：李乾[1,2,3] 蒋潇宇 王星星 王清良[1,2] 胡鄂明[1,2] 史文革[1,2] 

机构地区：[1]南华大学铀矿冶生物技术国防重点学科实验室,湖南衡阳421001 [2]南华大学核资源工程学院,湖南衡阳421001 [3]南华大学矿业工程博士后流动站,湖南衡阳421001

出　　处：《南华大学学报（自然科学版）》2017年第3期25-29,共5页Journal of University of South China：Science and Technology

基　　金：中国博士后科学基金(2014M562116);湖南省自然科学基金(14JJ6024);南华大学核资源与环境大学生创新训练中心创新实验项目

摘　　要：为了探究组合浸矿细菌菌群的氧化特性及其群落结构,构建4株浸矿细菌(Acidithiobacillus ferrooxidans ATCC 23270,Leptospirillum ferriphilum YSK,Acidithiobacills thiooxidans A01,Acidithiobacills caldus S1)的组合浸矿菌群.实验结果表明:此组合浸矿细菌菌群中的菌体具有较高的生物活性,表现出良好的的铁、硫氧化能力.并采用实时荧光定量PCR技术对菌群的群落结构进行了分析.结果表明:此体系中A.ferrooxidans ATCC 23270、A.thiooxidans A01是优势种群;L.ferriphilum YSK和A.caldus S1是劣势种群.In order to explore the oxidation characteristics and community structure of mixed bioleaching bacterial consortia,a bacterial consortium including four typical bioleaching strains（ Acidithiobacillus ferrooxidans ATCC 23270,Leptospirillum ferriphilum YSK,Acidithiobacills thiooxidans A01,Acidithiobacills caldus S1） was constructed.The re-sults show that strains in bioleaching bacterial consortium exert high biological activity and great oxidation ability to iron and sulfur.Microbial community structure was analyzed by real-time fluorescent quantitative PCR technology.The results show that A.ferrooxidans ATCC23270 and A. thiooxidans A01 are the dominant species,while the minor is L. ferriphilum YSK and A.caldus S1.

关 键 词：浸矿细菌菌群 铁/硫氧化 群落结构 

分 类 号：TD853.37[矿业工程—金属矿开采]