基于新型量子点荧光微球的氯霉素免疫层析试纸条的制备和应用  

Development of Quantum Dot Submicrobeads-based Fluorescent Immunochromatographic Test Strip for Rapid Detection of Chloramphenicol

作　　者：丁乔棋 李丽[1] 范文韬 吕亚楠 胡建华 闫丽萍 宋素泉[1] 

机构地区：[1]南京农业大学动物医学院,南京210095

出　　处：《分析化学》2017年第11期1686-1693,共8页Chinese Journal of Analytical Chemistry

基　　金：国家重点研发计划(Nos.2016YFD0501200;2016YFD0501009);中央高校基本业务费(No.Y0201500195);江苏省自然基金面上项目(No.BK20161452);广东省科技计划重点项目(No.2017B020202010);江苏高校优势学科建设工程资助项目资助~~

摘　　要：以羧基化CdTe/ZnSe量子点荧光微球为标记物,通过1-乙基-(3-二甲基氨基丙基)碳二亚胺/N-羟基琥珀酰亚胺(EDC/NHS)活化法将氯霉素(CAP)单克隆抗体与量子点荧光微球偶联制备荧光探针。氯霉素全抗原(CAP-HS-BSA)及羊抗鼠二抗分别喷涂硝酸纤维素膜,形成检测线(T线)和质控线(C线),组装成新型氯霉素量子点荧光微球免疫层析试纸条,建立了快速、定量检测牛奶中CAP的方法。本研究开发的量子点荧光微球试纸条可在15min内完成牛奶样品中CAP的定量检测,线性范围为0.1~100.0μg/L,检出限为0.1μg/L。牛奶样品CAP的加标回收率为93.3%~97.9%,相对标准偏差在4.9%~6.9%之间。A fluorescent immunochromatographic test strip based on the quantum dots submicrobeads（ QBs）was developed for quantitative detection of chloramphenicol（ CAP）. In this method,monoclonal antibody of CAP and OBs complex fluorescent probe was first prepared using 1-ethyl-3-（ 3-dimethylaminopropyl）carbodiimide/N-hydroxysuccinimide coupling approach,then complete antigen CAP-HS-BSA was synthesized and sprayed on nitrocellulose membrane as test line（ T line）. Similarly,goat anti-mouse antibody was sprayed as control line（ C line）. The time required for the analysis was 15 min,and the limit of detection（ LOD） for CAP was 0. 1 μg/L,with a working range of 0. 1-100 μg/L. In spiked milk samples,the test strip demonstrated high recoveries in the range from 93.3% to 97.9% with relative standard deviations of less than7%.

关 键 词：氯霉素 量子点微球 免疫层析试纸条 

分 类 号：O657.3[理学—分析化学] TS252.7[理学—化学]