二硫化二砷和靛玉红对骨髓增生异常综合征细胞MUTZ-1凋亡及其相关蛋白的影响  

Effects of As_2S_2 and indirubin on apoptosis and related proteins of apoptosis in MUTZ-1 cell of myelodysplastic syndrome

作　　者：杨秀鹏[1] 周庆兵[1] 麻柔[1] 王洪志[1] 胡晓梅[1] 许勇钢[1] 

机构地区：[1]中国中医科学院西苑医院血液科,北京100091

出　　处：《疑难病杂志》2017年第11期1132-1136,共5页Chinese Journal of Difficult and Complicated Cases

基　　金：国家自然科学基金资助项目(51803428);中国中医科学院自主选题研究项目(ZZ0708103)

摘　　要：目的研究中药青黄散主要成分二硫化二砷(As_2S_2)和靛玉红对骨髓增生异常综合征(MDS)细胞株MUTZ-1细胞周期、凋亡及凋亡相关蛋白Bcl-2,Bax和Caspase-3的影响。方法实验于2015年6月-2016年6月在中国中医科学院西苑医院血液科实验室进行。以MDS-RAEB细胞株MUTZ-1为研究对象,实验分4组:对照组、As_2S_2组、靛玉红组和联合组(As_2S_2和靛玉红联合应用)、以As_2S_2、靛玉红及二者联合分别作用MUTZ-1细胞48 h和72 h,以流式细胞术检测细胞周期,以Annexin V-FITC/PI法检测细胞的凋亡情况,以流式细胞术检测凋亡相关蛋白Bcl-2、Bax和Caspase-3的表达。结果 As_2S_2和靛玉红对MUTZ-1细胞有明显的抑制作用。与对照组相比,As_2S_2组和联合组在48 h时S期和G2/M期细胞的比例减少、G0/1期细胞比例增加(P均<0.05);在72 h时S期细胞的比例减少、G0/1期细胞比例增加(P均<0.01),G2/M期细胞的比例无明显变化(P=0.842)与对照组比较,靛玉红组细胞不同时间点各细胞周期无明显变化(P均>0.05)。与As_2S_2组和靛玉红组相比,联合组在48 h时S期和G2/M期细胞比例减少,G0/1期细胞比例增加(P均<0.01);72 h时S期细胞比例减少。G0/1期比例增加(P<0.01)。与对照组相比,As_2S_2组和靛玉红组细胞早期凋亡明显增加(P<0.05)。与As_2S_2组和靛玉红组相比,联合组促进早期凋亡作用也增强(P<0.05)。与对照组比较,靛玉红组和联合组在48 h时细胞表达Caspase-3增加(P<0.01);As_2S_2组在72 h时细胞表达Bcl-2减少(P<0.01)、Bax增加(P<0.05)。与As_2S_2、靛玉红组相比,联合组对MUTZ-1细胞Bcl-2、Bax和Caspase-3表达的无影响(P>0.05)。结论中药青黄散主要成分As_2S_2和靛玉红通过促进MDS细胞MUTZ-1的凋亡、影响凋亡相关蛋白起到治疗MDS的作用,单用As_2S_2可抑制MUTZ-1细胞的增殖,与靛玉红联合应用能增强其调控细胞周期、促进细胞凋亡的作用。Objective To investigate the effects of As2 S2 and indirubin of Qinghuangsan main ingredient on the cell cycle, apoptosis and apoptosis-related proteins Bcl-2, Bax and Caspase-3 in myelodysplastic syndromes( MDS) cell line MUTZ-1. Methods Experiment from June 2015 to June 2016 in China Academy of Chinese Medicine Xiyuan Hospital onset laboratories. With MDS-RAEB cell lines MUTZ-1 as the research object, the experiment including 4 groups : control group,group As2 S2, indirubin group and combined group( As_2 S_2 and indinubin joint application). To the As_2 S_2, indirubin and two joint role respectively MUTZ-1 48 h and 72 h, cells by flow cytometry to detect the cell cycle, with Annexin V-FITC/PI method to detect cell apoptosis, with flow cytometry to detect apoptosis related proteins the Bcl-2, Bax and Caspase 3-expression.Results As_2 S_2 and ndirubin for MUTZ-1 cells have obvious inhibitory effect. Compared with the control group, the As2 S2 group and combined group at 48 h S phase and G2/M phase cells to reduce, the proportion of G0/1 cell percentage increase(P < 0.05); The percentage of S phase cells during 72 h, I phase GO/cells percentage increase( P < 0.01), the proportion of G2/M phase cells has no obvious changes( P =0. 842). Group compared with control group, indirubin cells in different time points in each cell cycle has no obvious changes( P >0.05). And As_2 S_2 compared two groups and indirubin group, a joint group of at 48 h S period and G2/M phase cells proportion reduce G0/1 phase is increased( P < 0.05) 72 h G0/1 phase proportion increases( P <0.01). Compared with control group, the As_2 S_2 groups and indirubin early apoptosis cells increased significantly( P <0.05). With the As_2 S_2 and indirubin group,united group to promote early apoptosis effect also enhanced( P <0.05). Compared with control group, indirubin group and combined group at 48 h cells express Caspase-3 increase( P <0. 01), the As_2 S_2 group at 72 h cells expressing the Bcl-2 cut( P < 0. 01), increased Bax( P < 0. 05).Compared wi

关 键 词：二硫化二砷 靛玉红 骨髓增生异常综合征 MUTZ1细胞 凋亡 

分 类 号：R551.3[医药卫生—血液循环系统疾病]