饥饿和复投喂对金钱鱼四种igfbp基因表达的影响  

Effect of Hunger and Refeeding on the Expression of Scatophagus argus's Four igfbp Genes

作　　者：王妹 陈华谱[1,2] 江东能 李广丽 朱春华[1] 吴天利[1] 邓思平[1] 

机构地区：[1]广东海洋大学水产学院海洋生态与养殖环境湛江市重点实验室,广东湛江524088 [2]青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室,山东青岛266071

出　　处：《海南热带海洋学院学报》2017年第5期1-7,共7页Journal of Hainan Tropical Ocean University

基　　金：广东省自然科学基金(2016A030313743);广东省海洋与渔业局科技攻关与研发及技术推广项目(A201608B01;2017A0012);广东省教育厅特色创新项目(2015KTSCX058);湛江市财政资金科技竞争性分配项目(2016A03017);青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室开放课题(2016LMFS-B12)

摘　　要：本文通过实时荧光定量PCR检测了igfbp-1、igfbp-3、igfbp-4、igfbp-5基因在金钱鱼(Scatophagus argus)下丘脑、脑垂体、性腺、鳃、心脏、肾、肝、脾、肠、肌肉组织中的表达量及经过饥饿和复投处理后在肝脏中的表达量差异.结果显示:4种igfbp在金钱鱼11种组织中均有表达,其中igfbp-1在脑垂体和肝脏中表达量最高,在下丘脑和鳃中次之,在肾脏中最低.igfbp-3在脑垂体和肌肉中表达量最高,而在脾脏、胃、肾脏、肠中相对较低;igfbp-4在下丘脑和心脏中表达量最高,在其他组织中较低;igfbp-5在肠、鳃、脾中表达量最高,在肌肉、下丘脑、心脏、肾脏和肝脏中较低.饥饿2d后,金钱鱼肝脏中igfbp-1和igfbp-3基因的表达量组显著升高,而igfbp-4和igfbp-5基因与对照组相比无显著差异;饥饿7d后,肝脏中igfbp-1、igfbp-3、igfbp-4、igfbp-5基因的表达量均较对照组显著升高;在饥饿7d后复投,igfbp-1、igfbp-3、igfbp-4、igfbp-5基因表达量均显著降低.结果表明igfbp-1、igfbp-3、igfbp-4、igfbp-5在金钱鱼的摄食调节中起着重要作用.This paper detected by Real-time q PCR the expression of igfbp-1,igfbp-3,igfbp-4 and igfbp-5 genes of hypothalamus,pituitary,gonad,gill,heart,kidney,liver,spleen,intestine and muscle in Scatophagus argus,and the expression of those genes in liver processed by 2 d's and 7 d's starvation and re-feeding after 7 d's starvation.The results showed that the four genes were expressed differently in 11 tissues in Scatophagus argus,whereas,igfbp-1 has the highest expression in pituitary and liver,followed by the expression in hypothalamus and gill less,lowest expression in kidney.igfbp-3 has the highest expression in pituitary and muscle,while that in the spleen,stomach,kidney,intestine is relatively low.igfbp-4 has the highest expression in hypothalamus and heart,while that in the other tissues is low.igfbp-5 has the highest expression in the intestine,gill and spleen,while that in muscle,hypothalamus,heart,kidney and liver was low.After 2 d's starvation,the genes expression of igfbp-1 and igfbp-3 in the liver increased significantly,but that of igfbp-4 and igfbp-5 in the lever showed no obvious discrepancy with the control groups.After 7 d's starvation,the genes expression of igfbp-1,igfbp-3,igfbp-4 and igfbp-5 in the liver all increased significantly with contrast to the control groups.Refeeding after 7 d's starvation significantly reduced he genes expression of igfbp-1,igfbp-3,igfbp-4 and igfbp-5 in the liver.The results showed that igfbp-1,igfbp-3,igfbp-4 and igfbp-5 play an important role in the process of growth and metabolism of Scatophagus argus.

关 键 词：金钱鱼 类胰岛素生长因子结合蛋白 饥饿 复投喂 

分 类 号：S965[农业科学—水产养殖]