抗番茄黄化曲叶病基因ty-5序列变异及表达分析  

Sequence variation and expression analysis of tomato yellow leaf curl virus resistance gene ty-5

作　　者：姜静[1,2] 王银磊[1] 赵丽萍[1] 周蓉[1] 李亚茹[1,2] 余文贵[1] 赵统敏[1] 

机构地区：[1]江苏省农业科学院蔬菜研究所,江苏南京210014 [2]南京农业大学园艺学院,江苏南京210014

出　　处：《江苏农业学报》2017年第5期1105-1110,共6页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学青年基金项目(31401884);江苏省自然科学青年基金项目(BK20140739);江苏省农业科学院基本科研业务专项[ZX(15)4022]

摘　　要：番茄黄化曲叶病是番茄的主要病害之一,选育抗病品种是抵御该病害的重要途径。本研究对目前应用较少的抗番茄黄化曲叶病基因(ty-5)进行研究,对克隆片段进行序列分析,确定该基因在启动子区域和转录区域均存在碱基突变,并利用含有该基因的抗病材料AVTO1227对ty-5的表达进行分析,不同材料中ty-5及其等位基因Ty-5的表达量在接种番茄黄化曲叶病毒(TYLCV)前后均没有显著变化。本研究为下一步针对ty-5翻译区域内单核苷酸变异导致其功能变化的研究提供了依据,同时也为抗番茄黄化曲叶病育种提供了有效的连锁标记。Tomato yellow leal curl virus（TYLCV） disease is one of the important diseases of tomato. Breeding of re-sistant varieties was an important way^ to defense TYLCV disease. In this study, ty-5 gene which rarely reported was cloned and its fragment sequence was analyzed. Sequence mutation happened in the promoter region and the transcribed region of ty-5. Resistant material AVT01227,which containing the ty-5 gene,was used for expression analyzing of ty-5. The results showed that the expression level of ty-5 and Ty-5 did not have significant change before and after TYLCV inoculation in dif-ferent tomato materials. This study provided theoretical basis for the next step research of the single nucleotide in the tran-scription region of ty-5. In the meantime,linkage marker of ty-5 also provided an effective method for breeding.

关 键 词：番茄黄化曲叶病毒 抗番茄黄化曲叶病基因(ty-5) 碱基突变 表达分析 基因型鉴定 

分 类 号：S641.203.4[农业科学—蔬菜学]