破骨细胞在骨关节炎中的增殖变化及意义  

Proliferation change and significance of osteoclasts in osteoarthritis

作　　者：肖壮 唐涛[2,3] 朱成华 孙先润 李亚国[3] 李晓云 李莲娥[3] 

机构地区：[1]云南省第一人民医院药学部,昆明650034 [2]云南省第一人民医院康复科,昆明650034 [3]昆明理工大学医学院康复医学教研室,昆明650500 [4]云南新昆华医院药学部,昆明650300 [5]云南省第一人民医院骨科,昆明650034

出　　处：《重庆医学》2017年第30期4181-4184,共4页Chongqing medicine

基　　金：云南省应用基础研究计划项目(2013FZ182);昆明理工大学引进人才基金资助项目(KKZ3201460023)

摘　　要：目的探讨破骨细胞(OC)在骨关节炎(OA)各个时期中的增殖变化及临床意义。方法 20只健康成年SD雄性大鼠用改良Hulht手术方法造模,左膝为对照组,右膝为OA模型组。分别于术后1、2、4、8周时采集全膝关节(n=5),并置于4℃多聚甲醛(PFA)液固定,石蜡包埋切片,抗酒石酸酸性磷酸酶(TRAP)、甲苯胺蓝(TB)及番红-O快绿染色,观察软骨形态学改变并半定量TRAP染色阳性的OC数量,通过Mankin′s评价OA软骨破坏的进展,采用SPSS17.0统计软件进行统计分析。结果两组间OC均呈快速增高,然后下降的一过性增殖变化。对照组(左膝)在1周时OC数量为(65.20±4.12)个/mm^2,2、4周时逐渐减少,分别为(47.20±4.31)个/mm^2、(26.20±3.87)个/mm^2,8周时几乎看不到,细胞数为(7.00±2.28)个/mm^2;OA模型组(右膝)在1周时OC数量为(70.40±5.46)个/mm^2,2周时增加至(86.20±5.42)个/mm^2,4周时减少至(38.00±3.16)个/mm^2,8周时几乎看不到,细胞数为(6.21±2.93)个/mm^2。模型组OC数量在2、4周时明显高于对照组,差异有统计学意义(P<0.05)。结论大鼠膝关节OA实验的早中期OC大量增殖,OC可能参与了骨关节炎的形成。Objective To explore the proliferative changes and clinical significance of osteoclasts（OC）in various stages of osteoarthritis（OA）.Methods Twenty healthy adult male SD rats were made the model by modified Hulht procedure,the left knee served as the control group and the right knee as the OA model group.The total knee joint（n=5）was collected at postoperative 1,2,4,8 weeks,fixed at 4 ℃ 4% poly formaldehyde（PFA）liquid,embedded by paraffin for conducting sections,and stained by TRAP,toluidine blue（TB）and safranine O（Saf O）fast staining.Then the cartilage morphology change was observed and OC positive cells number with TRAP staining were semi-quantitatively detected,the OA cartilage destruction progression was evaluated by Mankin′s method and SPSS17.0 statistics software was used to conduct statistical analysis.Results OC in the two groups showed the transient change of rapidly increasing and then decreasing.The OC number at 1 week in the control group（left knee）was（65.20±4.12）cells/mm^2,and was gradually reduced at 2,4 weeks,which were（47.20±4.31）cells/mm and（26.20±3.87）cells/mm^2,which at 8 weeks was almost invisible,the number of cells was（7.00±2.28）cells/mm^2;the OC number at 1 week in the OA model group（right knee）was（70.40 ± 5.46）cells/mm^2,increased to（86.20±5.42）cells/mm^2 at 2 weeks,reduced to（38.0±3.16）cells/mm^2 at 4 weeks,was almost invisible at 8 weeks,the number of cells was（6.21±2.93）cells/mm^2.The OC number at 2,4 weeks in the model group was significantly higher than that in the control group,the difference had statistical significance（P〈0.05）.Conclusion Large numbers of osteoclasts are proliferated in the early and middle stages of rat knee osteoarthritis,which indicating that OC might be involved in the formation of osteoarthritis.

关 键 词：破骨细胞 骨关节炎 关节软骨 软骨下骨 关节损伤 

分 类 号：R684.3[医药卫生—骨科学]