叶绿素非离子表面活性剂囊泡的制备及其理化性质的表征  

Preparation of chlorophyll nonionic surfactant vesicles and characterization of their physical and chemical properties

作　　者：刘兵[1,2] 刘兆明 张春燕 王慧云[2] LIU Bing LIU Zhao-ming ZHANG Chun-yan WANG Hui-yun(School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250062, China College of Pharmaceutical Science, Jining Medical University, Rizhao 276826, China)

机构地区：[1]济南大学山东省医学科学院医学与生命科学学院,济南250062 [2]济宁医学院药学院,日照276826

出　　处：《中国新药杂志》2017年第20期2472-2477,共6页Chinese Journal of New Drugs

摘　　要：目的:制备叶绿素非离子表面活性剂囊泡(chlorophyll nonionic surfactant vesicles,Chl-NS),并对其理化性质进行表征。方法:以非离子表面活性剂脂肪酸山梨坦-80(Span-80)和聚山梨酯-80(Tween-80)复配并结合胆固醇为膜骨架,采用薄膜超声分散法制备叶绿素非离子表面活性剂囊泡;利用透射电镜和Malvern粒度仪分别考察其形态、粒径、分散系数、Zeta电位及稳定性;并进一步考察囊泡对叶绿素的包封率以及包载于囊泡中的叶绿素荧光强度的变化。结果:叶绿素非离子表面活性剂囊泡呈核壳层球形结构,大小均匀,平均粒径为(130.2±10.0)nm,多分散指数(polydispersity index,PDI)值为(0.240±0.01),Zeta电位为(-30.0±5.0)m V,对叶绿素包封率达到(45.2±2.1)%;叶绿素包载于囊泡后,体系的荧光强度明显增强且具有较高的稳定性。结论:本实验制备了稳定性高、粒径均匀、对叶绿素具有良好的包封作用,具有较强近红外荧光特征的非离子表面活性剂囊泡,该囊泡体系有望应用于体内成像系统的相关研究,具有重要临床意义。Objective： To prepare chlorophyll nonionic surfactant vesicles （Chl-NS） and study their physical and chemical properties.Methods： Chl-NS were prepared by thin-film ultrasonic dispersion method with Span-80,Tween-80 and cholesterol as membrane skeleton; the morphology of Chl-NS was observed by transmission electron microscope; the mean particle size,particle size distribution,Zeta potential and stability were measured by laser particle size analyzer; the vesicle encapsulation efficiency of chlorophyll and the changes of fluorescence intensity of vesicle-coated chlorophyll were determined by ultrafiltration-fluorescence spectrophotometry.Results： Chl-NS had spherical nuclear shell structure and were uniform in size with the mean particle size of （130.2 ± 10.0） nm,polydispersity index （PDI） of （0.240 ± 0.01）,Zeta potential of （-30.0 ± 5.0） m V and encapsulation efficiency of （45.2 ± 2.1） %; the fluorescence intensity of the vesicles-coated chlorophyll aqueous suspensions was larger than that of the chlorophyll aqueous suspensions alone,with high stability.Conclusion： Chl-NS were prepared successfully with narrow size distribution,good stability,and strong fluorescence by thin-film ultrasonic dispersion method.The system is expected to be applied in the research of in vivo imaging system and has important clinical significance.

关 键 词：叶绿素 囊泡 薄膜超声分散法 荧光分光光度法 稳定性 

分 类 号：R943[医药卫生—药剂学]