北柴胡侧根发育及影响因素探究  

Root development in Bupleurum chinense and its impact factors

作　　者：段莎莎[1] 侯大斌[1] 余马[1] 舒晓燕[1] 黄晶[1] 刘丹[1] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621000

出　　处：《中草药》2017年第19期4062-4067,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81603223);四川省"十三五"科技育种攻关项目(2016NYZ0020)

摘　　要：目的探索北柴胡Bupleurum chinense侧根发育过程及影响侧根发育的因素,为北柴胡产量提升及商品性改良提供技术支撑。方法对北柴胡品种川北柴1号主根进行石蜡切片观察侧根原基发育动态图,以及添加不同浓度生长调节物质后进行根系参数考察。结果北柴胡侧根原基起源于中柱鞘细胞,部分内皮层细胞也参与侧根原基发育;施加10μmol/L IAA和Ca2+浓度均最大化促进北柴胡根系发育,而浓度过高则抑制侧根发育;施加0.1μmol/L TIBA和10μmol/L EDTA显著促进柴胡根系发育,浓度过高则起抑制效果。结论本研究方法还可以广泛应用于人参、当归等以根入药的药用植物中,对药用植物栽培及育种工作具有重大意义。Objective To identificate the development process and impact factors of lateral root in Bupleurum chinense. MethodsThe dynamic growth of lateral root primordia was observed by applying paraffin sectioning, and by light microscopy. In addition, the influence of growth regulators treatment on lateral root growth was also investigated. Results The lateral root primordium formation of B. chinense started near the pericycle cells, part of the endodermis cells also participated in this formation; 10 μmol/L IAA and Ca2＋greatly promoted the development of lateral root, but its high concentration inhibited lateral root emergence; 0.1 μmol/L TIBA and 10 μmol/L EDTA promoted the development of lateral root, but its high concentration inhibited lateral root emergence. Conclusion This research method can also be applied in other pharmaceutical plants, such as Angelica sinensis and Panax ginseng. So the study is very important to medicinal plant cultivation and breeding.

关 键 词：北柴胡 侧根发育 中柱鞘 生长素 EDTA TIBA 

分 类 号：R282.21[医药卫生—中药学]