基于HPLC-Q-TOF/MS的六经头痛片化学成分分析  

Analysis of chemical constituents in Liujing Toutong Tablets by HPLC-Q-TOF/MS

作　　者：游飞祥 袁雪海 许浚[1,3] 朱晓丹[2] 张洪兵[3] 李冲冲 韩彦琪[3] 龚苏晓[3] 陈常青[3] 张铁军[3] YOU Fei-xiang YUAN Xue-hai XU Jun ZHU Xiao-dan ZHANG Hong-bing LI Chong-chong HAN Yan-qi GONG Su-xiao CHEN Chang-qing ZHANG Tie-jun(Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China Tianjin Zhongxin Pharmaceutical Group Co., Ltd., Longshunrong Pharmaceutical Factory, Tianjin 300457, China)

机构地区：[1]天津中医药大学,天津300193 [2]天津中新药业集团股份有限公司隆顺榕制药厂,天津300457 [3]天津药物研究院,天津300193

出　　处：《中草药》2017年第20期4157-4166,共10页Chinese Traditional and Herbal Drugs

摘　　要：目的运用高效液相色谱-四极杆-飞行时间质谱联用技术(HPLC-Q-TOF/MS),对六经头痛片中的化学成分进行分析鉴定。方法采用Diamonsil C_(18)(250 mm×4.6μm,5μm)反相HPLC梯度洗脱法分离各主要成分,电喷雾电离源正负离子模式下对色谱流出物进行高分辨四级杆飞行时间串联质谱检测,对各主要色谱峰进行归属;同时结合化合物准确相对分子质量、质谱碎片离子信息及相关文献数据分析鉴定化合物。结果通过质谱裂解规律结合自建的8味组方药材的化学成分库信息,共鉴定出95个化合物,主要包括异黄酮类、香豆素类、环烯醚萜类和苯酞类等成分,并对化合物的药材来源进行了归属。结论采用HPLC-Q-TOF/MS联用技术比较全面地阐明了六经头痛片的化学组成,为其药效物质基础研究和质量控制提供了参考。Objective To separate and identify the chemical constituents in Liujing Toutong Tablets（LTT） by HPLC-Q-TOF/MS. Methods The main components were separated by using the gradient elution method with RP-HPLC, Diamonsil C_（18）（250 mm × 4.6 μm, 5 μm）. The positive and negative electro spray ionization（ESI） source was adopted for determine the chromatographic effluent, the main chromatographic peaks are assigned and distinguished by Q-TOF. These components were further analyzed by accurately relative molecular mass of compounds, and Combined with Positive and negative ions MS information and related literature data. ResultsAccording to the MS principle, the corresponding reference substances and literature datas, Ninety-five compounds of LTT were identified, mainly including isoflavones, coumarins, iridoids and phthalides. Furthermore, all of the constituents were surveyed and classified according to their medicinal materials derivation. Conclusion In this study, the main components in LTT were elucidated completely by HPLC-Q-TOF/MS. The results could provide the evidence for the research on active constituents and quality control of LTT.

关 键 词：六经头痛片 高效液相色谱-四极杆-飞行时间质谱联用技术 异黄酮类 香豆素类 环烯醚萜类 苯酞类 

分 类 号：R284.1[医药卫生—中药学]