六经头痛片治疗大鼠偏头痛的作用及机制研究  

Effects and mechanisms of Liujing Toutong Tablets on migraine of rats

作　　者：胡金芳[1,2] 付合明 袁雪海 霍璇 安梦培[1,2] 冯玥 申秀萍 HU Jin-fang FU He-ming YUAN Xue-hai HUO Xuan AN Meng-pei FENG Yue SHEN Xiu-ping(Tianjin Institute of Pharmaceutical Research New Drug Evaluation Co., Ltd., Tianjin 300301, China Tianjin Engineering Research Center of Drug Preclinical Assessment Technology, Tianjin 300301, China Tianjin Zhongxin Pharmaceutical Group Co., Ltd., Longshunrong Pharmaceutical, Tianjin 300457, China)

机构地区：[1]天津药物研究院新药评价有限公司,天津300301 [2]天津市新药非临床评价技术工程中心,天津300301 [3]天津中新药业集团股份有限公司隆顺榕制药厂,天津300457

出　　处：《中草药》2017年第20期4187-4191,共5页Chinese Traditional and Herbal Drugs

基　　金：国家科技重大专项(2015ZX09501004);天津市科技计划项目(16PTGCCX00090)

摘　　要：目的探讨六经头痛片治疗偏头痛的作用及可能的机制。方法通过sc硝酸甘油复制大鼠偏头痛模型,观察大鼠搔头次数和搔头反应潜伏期;采用酶联免疫实验(ELISA)检测脑组织中5-羟色胺(5-HT)、去甲肾上腺素(NE)、多巴胺(DA)水平及血清中β-内啡肽(β-EP)、降钙素基因相关肽(CGRP)、内皮素(ET)的量,采用生化法测定血清中一氧化氮(NO)量及一氧化氮合酶(NOS)活性。结果模型组大鼠在sc硝酸甘油后1~3 min即出现搔头,且次数频繁。与模型组比较,六经头痛片1.4、0.7 g/kg组可显著减少偏头痛模型大鼠的搔头次数(P<0.001),并呈剂量相关性,能够明显延长大鼠搔头反应的潜伏期(P<0.01);六经头痛片1.4、0.7 g/kg组可显著升高模型大鼠血清β-EP、ET及脑内DA的量,显著降低血清CGRP的量和NOS活性,六经头痛片1.4 g/kg组可明显降低血清NO的量,对脑内5-HT、NE的量基本无影响。结论六经头痛片有显著的治疗偏头痛作用,可以升高血清β-EP、ET、DA的量,降低CGRP、NO的量及NOS活性,其作用机制与收缩血管及中枢镇痛有关。Objective To investigate effect and mechanisms of Liujing Toutong Tablets（LTT） on migraine model rats. MethodsRats were sc injected with nitroglycerin to establish migraine model, effect of LTT on scratching latency and frequency of migraine model rats was investigated; 5-hydroxytryptamine（5-HT）, norepinephrine（NE）, and dopamine（DA） in brain tissue were detected; And β-endorphin（β-EP）, calcitonin gene related pepitde（CGRP）, and endothelin（ET） in serum were examined by ELISA; The content of nitric oxide（NO） and activity of nitric oxide synthase（NOS） in serum were examined. Results After sc injected with nitroglycerin in 1 to 3 min, the rats in model group began to scratch its head frequently, scratching latency of rats in high-and low-dose（1.4 and 0.7 g/kg） of LTT group was extended（P〈0.01） and scratching frequency was decreased（P〈 0.001）; The content of β-EP and ET in serum and DA in brain tissue of rats in high-and low-dose（1.4 and 0.7 g/kg） of LTT group were increased（P〈0.05, 0.01, 0.001）, and the content of CGRP and the activity of NOS in serum were decreased（P〈0.05）. The content of 5-HT and NE in brain tissue of rats in high-and low-dose（1.4 and 0.7 g/kg） of LTT group had no significant change. Conclusion LTT has a significant therapeutic effect on migraine, and could inhibit contractile response of vascular. The mechanisms may be connected with increasing content of β-EP, ET, DA and decreasing content of CGRP and the activity of NOS.

关 键 词：六经头痛片 偏头痛 硝酸甘油 镇痛 降钙素基因相关肽 内皮素 Β-内啡肽 一氧化氮 

分 类 号：R285.5[医药卫生—中药学]