六经头痛片对离体大鼠主动脉收缩及三叉神经降钙素基因相关肽表达的影响  

Effects of Liujing Toutong Tablets on isolated vascular smooth muscle and expression of calcitonin gene-related peptide in trigeminus

作　　者：邸志权[1,2] 安梦培 朱晓丹 冯玥[1,2] 霍璇[1,2] 付合明 胡金芳[1,2] 申秀萍 DI Zhi-quan AN Meng-pei ZHU Xiao-dan FENG Yue HUO Xuan FU He-ming HU Jin-fang SHEN Xiu-ping(Tianjin Institute of Pharmaceutical Research New Drug Evaluation Co., Ltd., Tianjin 300301, China Tianjin Engineering Research Center of Drug Preclinical Assessment Technology, Tianjin 300301, China Tianjin Zhongxin Pharmaceutical Group Co., Ltd., Longshunrong Pharmaceutical, Tianjin 300457, China)

机构地区：[1]天津药物研究院新药评价有限公司,天津300301 [2]天津市新药非临床评价技术工程中心,天津300301 [3]天津中新药业集团股份有限公司隆顺榕制药厂,天津300457

出　　处：《中草药》2017年第20期4192-4197,共6页Chinese Traditional and Herbal Drugs

基　　金：国家科技重大专项(2015ZX09501004);天津市科技计划项目(16PTGCCX00090)

摘　　要：目的观察六经头痛片对大鼠离体血管平滑肌收缩活动及三叉神经降钙素基因相关肽(CGRP)表达的影响。方法制备大鼠离体主动脉血管环,采用麦氏浴槽法观察六经头痛片对血管平滑肌收缩活动的影响;培养三叉神经节组织,观察六经头痛片对三叉神经节CGRP表达的影响。结果六经头痛片可以减弱KCl及去甲肾上腺素(NE)引起的胸主动脉环收缩反应,对KCl收缩的半数抑制浓度(IC_(50))为0.97 mg/m L,对NE收缩的IC_(50)为0.55 mg/m L。而且,六经头痛片在1.4、0.14mg/m L时可明显减少离体培养24、48 h后三叉神经节CGRP阳性细胞的数量(P<0.05)。结论六经头痛片既可以对抗血管平滑肌的收缩,又可以降低三叉神经具有扩张血管作用的CGRP的量,说明六经头痛片具有稳定血管舒缩的作用。Objective To investigate the effects of Liujing Toutong Tablets（LTT） on isolated vascular smooth muscle and the expression of calcitonin gene-related peptide（CGRP） in trigeminus. Methods Rats＇ thoracic aorta and trigeminus were isolated to investigate the exact effect by measuring the contractile response of vascular and the expression of CGRP in trigeminus. Results The LTT could reduce the quantity of positive CGRP cells expressed in trigeminus（P〈0.05） and weaken the contractile response of thoracic aorta induced by KCl and NE, and the IC50 is 0.97 mg/mL and 0.55 mg/mL, respectively. Conclusion LTT could inhibit contractile response of vascular smooth muscle, meantime, reduce the quantity of positive CGRP cells expressed in trigeminus. The results show that LTT can stabilize vascular systaltic property.

关 键 词：六经头痛片 偏头痛 三叉神经 降钙素基因相关肽 速激肽受体1 

分 类 号：R285.5[医药卫生—中药学]