IL-12p40与IL-12p70在单病原菌性血流感染小鼠模型中的表达及意义  

Expressions and significances of IL-12p40 and IL-12p70 in single pathogen bloodstream infection in mice model

作　　者：杨明[1,2] 麻雅婷 何赏[1] 王佳楠 陈琛[1] 张可昕[1,2] 沙英娇 王成彬[1,2] YANG Ming MA Ya-ting HE Shang WANG Jia-nan CHEN Chen ZHANG Ke-xin SHA Ying-jiao WANG Cheng-bin(Chinese PLA General Hospital, Beijing 100853, China)

机构地区：[1]解放军总医院临床检验科,北京100853 [2]温州医科大学检验医学院生命科学学院,浙江温州325035

出　　处：《中华医院感染学杂志》2017年第20期4614-4617,共4页Chinese Journal of Nosocomiology

基　　金：国家重大科学仪器专项基金资助项目(2012YQ18011708)

摘　　要：目的探讨白细胞介素-12p40(IL-12p40)和IL-12p70在不同病原菌致小鼠血流感染模型中的动力学变化及意义。方法建立金黄色葡萄球菌、粪肠球菌、大肠埃希菌和肺炎克雷伯菌标准菌株的CD-1(ICR)小鼠血流感染模型并评价;采用蛋白液相芯片检测技术测定各实验组与磷酸缓冲溶液(PBS)的对照组0.5、1、3、6、12、24和48h的IL-12p40和IL-12p70浓度。结果金黄色葡萄球菌组与对照组相比,IL-12p40的浓度稍升高,而粪肠球菌组、大肠埃希菌组和肺炎克雷伯菌组感染后1h即明显上升,3h时达峰值,分别为(216.36±50.63)pg/mL、(118.32±9.98)pg/mL和(129.19±27.11)pg/mL。感染12h后仍维持较高水平,与对照组相比差异有统计学意义(P<0.05);金黄色葡萄球菌组IL-12p70浓度在感染后6h时明显升高且达峰值,为(389.31±118.07)pg/ml;粪肠球菌组IL-12p70浓度在感染后3h明显升高,与对照组相比差异有统计学意义(P<0.05),大肠埃希菌组与肺炎克雷伯菌组IL-12p70浓度于感染后3h达峰值,分别为(42.20±3.54)pg/mL和(62.80±8.37)pg/mL但升高幅度显著低于金黄色葡萄球菌组和粪肠球菌组(P<0.05)。结论 IL-12p40和IL-12p70在四组单病原菌性血流感染模型早期均明显升高,感染金黄色葡萄球菌或粪肠球菌后IL-12p70浓度的升高程度明显高于大肠埃希菌或肺炎克雷伯菌感染。联合检测IL-12p40和IL-12p70等指标对区分革兰阴阳性菌感染有一定价值。OBJECTIVE To investigate the dynamic changes and significance of IL-12 p40 and IL-12 p70 in bacterial bloodstream infection caused by different pathogens in mice model.METHODS Four models of bloodstream infection of S.aureus,E.faecalis,E.coli and K.pneumoniae in ICR mice were established and evaluated.The contents of IL-12 p40 and IL-12 p70 in experimental group and control groups of PBS solution at 0.5 h,1 h,3 h,6 h,12 h,24 hand 48 hwere detected by Luminex platform.RESULTS The concentration of IL-12 p40 was slightly increased in S.aureus group compared with control group,but increased significantly in E.faecalis group,E.coli group and K.pneumoniae group after 1 hof infection,and reached the highest value at 3 hafter the infection,the peak concentration were（216.36±50.63）pg/mL,（118.32±9.98）pg/mL and（129.19±27.11）pg/ml respectively,and remained at high values after 12 hof infection,and the differences were significant compared with control group（P〈0.05）.The content of IL-12 p70 in S.aureus group increased significantly and reached the maximum value after 6 h of infection,and its peak concentration was（389.31±118.07）pg/mL.The content of IL-12 p70 in E.faecalis group increased significantly after 3 h of infection,and the difference was significant compared with control group（P〈0.05）.The contents of IL-12 p70 in E.coli group and K.pneumoniae group reached the maximum value after 3 hof infection,which were（42.20±3.54）pg/mL and（62.80±8.37）pg/mL,but the increasing ranges were significantly less than those in S.aureus group and E.faecalis group（P 0.05）.CONCLUSION IL-12 p40 and IL-12 p70 are significantly increased in the 4 bloodstream infection models at the early stage of infection.After the infection with S.aureus or E.faecalis,the concentrations of IL-12 p70 is significantly higher than that of E.coli or K.pneumoniae infection.The combined detection of IL-12 p40 and IL-12 p70 is of some value in differentiating gram-negative and gram-positive bacteria infections.

关 键 词：血流感染 小鼠模型 白细胞介素-12p40 白细胞介素-12p70 

分 类 号：R378[医药卫生—病原生物学]