土壤因子对海洋多粘类芽胞杆菌L_1-9在黄瓜根表土壤定殖的影响  

Effects of Soil Factors on the Colonization of Marine Biocontrol Bacterium Paenibacillus polymyxa L_1-9 in Cucumber Root Surface Soil

作　　者：王淑芳[1] 汪晶晶[1] 曹雪梅 李欢[1] 王琦[1] 王军强[1] 马桂珍[1] 暴增海[1] WANG Shufang WANG Jingjing CAO Xuemei LI Huan WANG Qi WANG Junqiang MA Guizhen BAO Zenghai(Jiangsu Key Laboratory of Marine Biotechnology/College of Marine Life and Fisheries, Huaihai Institute of Technology, Lianyungang 222005, Chin)

机构地区：[1]淮海工学院海洋生命与水产学院/江苏省海洋生物技术重点实验室,连云港222005

出　　处：《中国生物防治学报》2017年第5期675-680,共6页Chinese Journal of Biological Control

基　　金：江苏省自然科学基金(BK20141248);连云港市科技局现代农业项目(NYYQ1619;CN1514);淮海工学院青年基金项目(Z2014005)

摘　　要：本试验考察了土壤温度、有机质含量和pH对海洋生防细菌多粘类芽胞杆菌L_1-9在黄瓜根表土壤中定殖的影响。采用实时荧光定量PCR方法检测了不同时期黄瓜根表土壤中菌株L_1-9的16S r DNA拷贝数。结果表明,土壤有机质含量、土壤温度和pH对菌株L_1-9在黄瓜根表的定殖有显著影响(P<0.01)。25℃定殖数量最高,为5.79×108拷贝/g土,是15℃时的3.4倍;添加有机肥20~40 g/kg土壤,可以促进菌株L_1-9在黄瓜根际定殖;pH 7时,菌株L_1-9在黄瓜根表土壤中的定殖数量最高。本研究为海洋生防菌L_1-9防治黄瓜土传病害提供了理论依据。In this study, the effects of soil organic matter contents, temperature and soil pH value on colonization of marine biocontrol bacterium of L1-9 in root surface soil were investigated. 16 S r DNA gene fragments of the strain in the root surface soil were quantified using quantitative real-time PCR method. The results showed that soil organic matter content, temperature and pH exhibited significant impacts on colonization of the strain L1-9 in root surface soil（P0.01）. The propagation of the strain L1-9 in root surface soil was faster under the temperature of 25 ℃, which was 5.79×108 copies per gram dry soil, and 4.3 times of that under 15 ℃. Addition of organic matter（20―40 g/kg） increased the population density of the strain L1-9. The maximum colonization level of the strain L1-9 in the root surface soil was achieved in pH 7. This study laid a foundation for the development and application of the marine biocontrol bacterium L1-9 against soilborne diseases of cucumber.

关 键 词：多粘类芽胞杆菌 根表土壤 定殖 实时荧光定量PCR 

分 类 号：S476[农业科学—农业昆虫与害虫防治]