列当生防菌层出镰刀菌Br-1发酵条件的优化及田间防治效果  

Optimization of Fermentation Medium Components and Conditions of Fusarium proliferatum Br-1 and Its Field Control Efficacy

作　　者：王亚娇[1] 纪莉景[1] 栗秋生[1] 肖颖[1] 王连生[1] 孔令晓[1] WANG Yajiao JI Lijing LI Qiusheng XIAO Ying WANG Liansheng KONG Lingxiao(Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture/IPM Center of Hebei Province/Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, China)

机构地区：[1]河北省农林科学院植物保护研究所/河北省有害生物综合防治工程技术研究中心/农业部华北北部作物有害生物综合治理重点实验室,保定071000

出　　处：《中国生物防治学报》2017年第5期692-698,共7页Chinese Journal of Biological Control

基　　金：河北省财政项目(F13R10001);河北省财政基本科研业务费项目(494-0401-JBN-6440)

摘　　要：列当是根寄生杂草,很难防除。菌株Br-1是从感病列当中分离出的对列当有较高防治效果的层出镰刀菌。为了提高菌株Br-1的产孢量,本研究通过单因素试验和正交设计试验对其培养基和发酵条件进行了优化。结果表明,菌株Br-1适宜的发酵培养基为葡萄糖1%,NH4NO_3 0.2%,KH_2PO_4 0.3%,Mg SO4·7H_2O0.1%;优化后的培养条件为:最初pH 5.0,培养温度24℃,装液量20%,振荡速度190 r/min,发酵时间6 d。较优化前的初始培养基和培养条件产孢量提高了257.5倍。在田间防治试验中菌株Br-1对弯管列当的防治效果最高可达67.2%。Biological control agent Br-1 was isolated from diseased broomrape and identified as Fusarium proliferatum. In order to increase the sporulation of strain Br-1, the medium components and fermentation conditions were optimized by single factor and orthogonal design. The results indicated that optimized medium components of strain Br-1 were glucose 1%, NH4NO3 0.2%, KH2PO4 0.3%, MgSO4·7H2O 0.1%. Optimum fermentation conditions of strain Br-1 were pH 5.0, medium capacity 50 m L/250 m L, temperature 24 ℃, rotating speed 190 r/min, fermentation time 6 d, with sporulation of Br-1 increased by 257.5 times in comparison with that under unoptimized conditions. Field trails showed that control efficacy of Br-1 on Orobanche cernua Loefling was up to 67.2%.

关 键 词：层出镰刀菌 发酵优化 培养基 防治效果 

分 类 号：S476[农业科学—农业昆虫与害虫防治]