2016年～2017年我国华北地区某规模化猪场经典美洲型猪繁殖与呼吸综合征病毒的分离鉴定及全基因组序列分析  

Isolation,characterization and complete genome analysis of classical North American type-porcine reproductive and respiratory syndrome virus in a large-scale pig farm in North China during 2016-2017

作　　者：刘春晓[1] 张洪亮[1] 张文立 相丽润 李真[1] 冷超粮[2] 陈家锃 彭金美[1] 王倩[1] 安同庆[1] 童光志[4] 蔡雪辉[1] 田志军[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150069 [2]南阳师范学院,河南南阳473061 [3]山东信得生物科技有限公司,山东青岛266100 [4]中国农业科学院上海兽医研究所,上海200241

出　　处：《中国预防兽医学报》2017年第9期691-696,共6页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(31270045;31502097)

摘　　要：为了解近期我国华北地区猪繁殖与呼吸综合征病毒(PRRSV)的流行及变异情况,本研究对该地区某猪场采集33份2016年~2017年疑似患PRRS猪病料样品进行PCR检测,对阳性样品进行病毒分离,并从分离株中选择两株病毒(SD158和SD192)进行全基因的序列扩增及分析。结果显示,检测到13份PRRSV阳性病料样品,病原为经典美洲型PRRSV,但仅分离到11株病毒(4株感染MARC-145细胞,另外7株感染PAM细胞)。SD158和SD192株与Resp PRRS MLV株(疫苗株)的核苷酸同源性最高,分别为99.7%和99.4%。对这两株PRRSV与VR-2332、Resp PRRS MLV的各基因的推导氨基酸序列进行分析,结果显示在VR-2332与Resp PRRS MLV株22处差异氨基酸中,SD158株有15处、SD192株有14处氨基酸和Resp PRRS MLV株相同,但二者仅各有6处及7处氨基酸与VR-2332株一致,在鉴别PRRSV VR-2332株与Resp PRRS MLV株的7处可能与毒力相关的氨基酸位点时发现,SD158与SD192株各有5处基酸与VR-2332株相同。根据以上结果推测SD158与SD192株可能来源于PRRSV Resp PRRS MLV株,具有上述分子变化特征的PRRSV在我国的流行状况值得关注。To investigate the prevalence and variationof porcine reproductive and respiratory syndrome virus （PRRSV） in North China, 33 clinical samples for PRRSV detection were collected from the suspected PRRSV-infected pigs in a pig farm of North China during 2016 to 2017. The results showed that 13 samples were positive for classical North American type-PRRSV, but only 11 strains were isolated （4 strains could infect MARC-145 cells and 7 could infect PAM cells）. The complete genomes of two isolates （SD158 and SD192） were sequenced, and had the highest genome nucleotide homology with RespPRRS MLV, which was 99.7% and 99.4%, respectively. There were 22 amino acid residues differences between RespPRRS MLV and VR-2332, of which 15 and 14 amino acid residues were consistent with RespPRRS MLV in SD158 and SD192 strains, respectively. There were 6 and 7 amino acid residues which were consistent with VR-2332 in SD158 and SD192 strains, respectively. In addition, seven amino acids related to virulence were identified in previous studies, while five of them in SD158 and SD192 isolates were consistent with VR-2332. Collectively, these data indicated that SD158 and SD192 were likely to be derived from RespPRRS MLV. The prevalence of PRRSV with above molecular characteristics in China is worthy of attention.

关 键 词：经典猪繁殖与呼吸综合征病毒 病毒分离鉴定 流行病学 

分 类 号：S852.65[农业科学—基础兽医学]