猪氨基肽酶N不是猪德尔塔冠状病毒入侵宿主细胞的受体  

Porcine Amino peptidase N(pAPN) is not a cellular receptor for porcine deltacoronavirus entry cell

作　　者：卢曼曼 张家林[1] 王洪峰[2] 时洪艳[1] 张鑫[1] 袁婧[1] 石达[1] 刘建波[1] 陈建飞[1] 冯力[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/猪消化道传染病创新团队,黑龙江哈尔滨150069 [2]哈尔滨维科生物技术开发公司,黑龙江哈尔滨150069

出　　处：《中国预防兽医学报》2017年第9期701-706,共6页Chinese Journal of Preventive Veterinary Medicine

基　　金：黑龙江省自然科学基金(C2016065);国家重点研发计划项目(2016YFD0500705;2016YFD0500103);黑龙江省科研机构创新能力提升专项(YC2015D011)

摘　　要：猪德尔塔冠状病毒(PDCoV)是一种新出现的冠状病毒,能够引起猪只发生呕吐和水样腹泻,临床症状与猪流行性腹泻(PED)和猪传染性胃肠炎(TGE)相似。相关研究表明猪氨基肽酶N(pAPN)是PED病毒(PEDV)和TGE病毒(TGEV)入侵宿主细胞的功能性受体。为研究pAPN是否是PDCoV入侵宿主细胞的受体,本研究以TGEV为指示病毒,通过BHK-pAPN细胞感染试验、pAPNRNA干扰试验、ST细胞过表达pAPN试验、可溶性pAPN阻断试验,发现BHK-pAPN细胞是PDCoV非易感细胞,在易感细胞ST上沉默和过表达pAPN对PDCoV的增殖并无影响,可溶性pAPN不能阻止PDCoV感染ST细胞。这些研究结果表明pAPN不是PDCoV入侵宿主细胞的受体,而且对PDCoV的增殖无影响。Porcine deltacoronavirus （PDCoV） is a novel coronavirus which can cause vomiting and watery diarrhea in pigs. Clinical symptoms of PDCoV infection are similar to those of porcine epidemic diarrhea （PED） and transmissible gastroenteritis （TGE）. Previous studies indicate porcine aminopeptidase N（pAPN） is a functional receptor for PEDV and TGEV entry cell. Does pAPN have a similar function to PDCoV invading host cells？ In this study, BHK-pAPN cell infection test, silence and overexpression of pAPN in ST cells, soluble pAPN blocking assay were used to confirm whether pAPN was a receptor for PDCoV using TGEV as a positive control. We found BHK-pAPN ceils failed to support PDCoV infection, but were susceptible to infection by TGEV. pAPN overexpression and siRNA of pAPN in ST cells affect-ted propagation of TGEV, but not PDCoV. Contrasting to TGEV, PDCoV did not bind soluble pAPN, and infection of ST cells was not inhibited by the soluble form of pAPN. These results indicated that pAPN was not a receptor for PDCoV invadring cell and had no affection on propagation of PDCoV in ST cells.

关 键 词：猪德尔塔冠状病毒 猪氨基肽酶N 受体 

分 类 号：S852.65[农业科学—基础兽医学]