我国部分地区牛源金黄色葡萄球菌基因多态性分型研究  

Identification of genetic polymorphism of Staphylococcus aureus isolated from bovines mastitis in parts of China

作　　者：武中庸 倪春霞[1] 赵吴静 徐进强 蒲万霞 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所中国农业科学院新兽药工程重点开放实验室/甘肃省新兽药工程重点实验室,甘肃兰州730050 [2]西北民族大学生命科学与工程学院,甘肃兰州730030 [3]甘肃农业大学动物医学学院,甘肃兰州730070

出　　处：《中国预防兽医学报》2017年第9期758-760,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：中央级公益性科研院所基本科研业务费专项资金项目-奶牛养殖环境中耐药数据库及奶牛乳房炎病原菌种库建设(610322016014);国家重点研发计划"畜禽重要病原耐药性检测与控制技术研究"(2016YFD0501306)

摘　　要：为了解我国牛源金黄色葡萄球菌(S.aureus)的基因多态性,本研究利用随机引物多态性扩增(RAPD)体系对174株分离自贵州、内蒙古、四川、上海、甘肃等地奶牛乳房炎的S.aureus及一株标准菌CVCC2246进行基因分型。结果表明,175株S.aureus均得到清晰的RAPD指纹图谱,扩增产物为1~9个片段,产物大小为240 bp^4 500 bp。所有菌株共分为8个基因型,其中1型16株;2型和3型各37株;4型15株;5型18株;6型14株;7型13株;8型8株。2型和3型菌株占总菌株42%以上,在贵州、内蒙古、四川、上海、甘肃分布广泛,为流行优势基因型;但5型是内蒙古地区的流行优势基因型。各地区菌株基因型比例有明显差异,可能与奶牛饲养水平和环境差异有关。In order to-know the gene polymorphisms of dairy sourced Staphylococcus aureus, 174 strains S.aureus isolated from dairy cows with mastitis in Guizhou, Inner Mongolia, Sichuan, Shanghai, Gansu and a standard strain were analyzed by random amplified polymorphic DNA （RAPD） in this study. The result showed that the amplified products bands were 1 to 9 fragments and the product sizes was 240 bp to 4 500 bp. Genotyping of these isolates indicated that all isolates were distributed into 8 different genotype. Among 175 isolates, 16 were type 1, 37 were type 2 and type 3 respectively, 15 were type 4, 18 were type 5, 14 were type 6, 13 were type 7 and type 8, 8 were type 8. Both of type 2 and type 3 were the dominant genotypes which were widely distributed in Guizhou, Shanghai, Sichuan, and Gansu provinces. Type 5 was the dominant genotype in Inner Mongolia. The proportion of 8 genotypes in different places were significant diffrence, which could be related to the feeding level and environment difference of dairy cow.

关 键 词：奶牛乳房炎 金黄色葡萄球菌 RAPD 基因型 

分 类 号：S852.61[农业科学—基础兽医学]