马波沙星在猪多杀性巴氏杆菌感染组织液中药代动力学的研究  

Pharmacokinetics of marbofloxacin in tissue cage fluid of pigs infected with Pasteurella multocida

作　　者：曾庆林[1] 苏佳 万鹏 裴鹏飞 韩东东 曾振灵[1] 

机构地区：[1]华南农业大学兽医学院广东省兽药研制与安全性评价重点实验室,广东广州510642

出　　处：《中国兽医科学》2017年第10期1326-1330,共5页Chinese Veterinary Science

基　　金：国家自然科学基金面上项目(31372480)

摘　　要：本试验通过建立组织笼感染模型,研究马波沙星在多杀性巴氏杆菌感染猪组织液中的药代动力学。向组织笼中注射0.5 mL对数期多杀性巴氏杆菌(5×10~8CFU/mL),感染后第24小时,对猪按体重单剂量肌内注射1.0 mg/kg和2.5 mg/kg马波沙星,按预定的时间点采集组织液样品,采用高效液相色谱(HPLC)测定组织液中马波沙星的浓度。用Win Nonlin5.2.1软件提供的非房室模型处理组织液药物浓度-时间数据。结果,不同剂量给药的主要药物动力学参数如下:1 mg/kg剂量给药:t_(max)=4 h±1.41 h,C_(max)=0.30 g/mL±0.06g/mL,AUC=9.96 g/mL·h±1.54 g/mL·h,t_(1/2β)=22.92 h±6.83 h;2.5 mg/kg剂量给药:t_(max)=5 h±1.41 h,C_(max)=0.71 g/mL±0.09 g/mL,AUC=21.06 g/mL·h±3.69 g/mL·h,t_(1/2β)=20.91 h±8.57 h。结果表明,马波沙星在多杀性巴氏杆菌感染猪组织液中的峰浓度较高,达峰时间较慢,消除半衰期较长。To study the pharmacokinetics of marbofloxacin in the tissue cage fluid of pigs infected with Pasteurella multocida,a tissue cage infection model was included in this study.Two dosages（1.0 mg/kg and 2.5 mg/kg） of marbofloxacin were administrated intramuscularly after 0.5 mL of P. multocida saline suspension was injected into tissue-cages for 24 h,and tissue cage fluid samples were sampled as designed scheme.Marbofloxacin concerntration in tissue cage fluid samples was determined by the HPLC.Concentration-time data were analysised with the non-compartment model offered by Win Nonlin software.In result,the main pharmacokinetic parameters acquired from different dosages were described as below,1 mg/kg：t_（max）=4 h±1.41 h,C_（max）=0.30 g/mL±0.06 g/mL,AUC=9.96 g/mL·h±1.54 g/mL·h,t_（1/2β）=22.92 h±6.83 h;2.5 mg/kg：t_（max）=5 h±1.41 h,C_（max）=0.71 g/mL±0.09 g/mL,AUC=21.06 g/mL·h±3.69 g/mL·h,t_（1/2β）=20.91 h ±8.57 h. In conclusion,marbofloxacin is absorbed and eliminated slowly in tissue cage fluid of pigs infected with P.multocida,and can reach high peak concentration.

关 键 词：马波沙星 药代动力学 多杀性巴氏杆菌 组织液 

分 类 号：S859.796[农业科学—临床兽医学]