不同产地壮药夜香牛中木犀草素的含量测定  

Determination of Luteolin in the Zhuang Medicine Vernonia cinerea(L.) Less. from Different Habitats

作　　者：谭小明[1] 周小雷[1] 周雅琴[1] 赵以民[1] 余丽莹[1] TAN Xiaoming ZHOU Xiaolei ZHOU Yaqin ZHAO Yimin YU Liying(National Engineering Laboratory of Southwest Endangered Medicinal Resources Development, National Development and Reform Commission, Guangxi Botanical Garden of Medicinal Plant, Nanning 530023, Chin)

机构地区：[1]西南濒危药材资源开发国家工程实验室/广西药用植物园,广西南宁530023

出　　处：《中国民族民间医药》2017年第20期18-20,共3页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：广西重大专项计划资助项目(桂科重1598005-10);国家自然科学基金资助项目(81360682);广西科学基金资助项目(2015GXNSFDA139012;2016GXNSFBA380007)

摘　　要：目的:建立高效液相色谱法(HPLC)测定广西不同产地壮药夜香牛中木犀草素的含量。方法:采用HPLC法对10个不同产地的夜香牛药材中木犀草素的含量进行检测。采用Alltima C18柱(4.6 mm×250 mm,5μm);以甲醇-乙腈(1∶1)为流动相A;0.5%磷酸为流动相B;流速:1.0 m L/min;柱温:30℃;检测波长:350 nm。结果:广西10个不同产地夜香牛药材中木犀草素含量在0.00200%~0.00997%之间。结论:本实验方法过程简单、测定结果可靠、且检测的重复性好,可作为研究壮药夜香牛质量标准的方法。Objective To determinate the content of luteolin in Zhuang medicine Vernonia cinerea （ L. ） Less. from different habi- tats of Guangxi by HPLC. Methods The luteolin in ten different habitats of V. cinerea was dominated by HPLC using Alltima C18 chro- matographic column （4. 6 mm × 250 mm, 5μm）. Methanol- Acetonitrile （1：1） and 0. 5% phosphoric acid were used as mobile phase, which flowing at 1.0 mL min-1 （with the gradient elution at 30 % -60 %, 0 to 30 min ）. The detection wavelength was 350 nm and column temperature was at 30 ℃. Results The content of luteolin in ten different habitats of V. cinerea was 0. 0020 % 0. 00997 %. Conclusion The HPLC method for the determination of luteolin contain in V. cinerea is simple, accurate and reproduci- ble, which may be used for the study quality standard of V. cinerea.

关 键 词：壮药 夜香牛 木犀草素 高效液相色谱法 产地 

分 类 号：R284.1[医药卫生—中药学]