HPLC法同时测定水栀子中栀子苷和西红花苷-Ⅰ的含量  

Simultaneous Determination of Geniposide and Crocin-Ⅰ in the Fruits of Gardenia jasminoides var. radicans by HPLC

作　　者：赵江平 牛忻 杨群 李淑娟 韦凤爽 郝丽莉[1] 刘江云[1] ZHAO Jiangping NIU Xin YANG Qun LI Shujuan WEI Fengshuang HAO Lili LIU Jiangyun(College of Pharmaceutical Sciences, SooChow University, Suzhou 215123, China Heze City Food and Drug Inspeetion and Research Institute, Heze 274000, China)

机构地区：[1]苏州大学医学部药学院,江苏苏州215123 [2]菏泽市食品药品检验检测研究院中药科,山东菏泽274000

出　　处：《中国民族民间医药》2017年第20期35-37,41,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：全国药学专业学位研究生优秀教学案例项目(2016工业药学);苏州大学医学部学生课外科研项目(2016-29)

摘　　要：目的:建立水栀子中两种主要组分的双波长同时含量测定方法。方法:采用Cosmosil 5C18-AR-Ⅱ色谱柱(250mm×4.6mm,5μm);流动相为甲醇–0.1%乙酸溶液梯度洗脱;流速1.0m L/min;检测波长238nm(1)和440nm(2),测定水栀子中栀子苷(1)、西红花苷-Ⅰ(2)的含量。结果:两种组分色谱分离良好,栀子苷、西红花苷-Ⅰ的线性范围分别为50.16~501.6μg/m L(r=0.9999)、10.13~101.3μg/mL(r=0.9997),加样回收率分别为97.77%、97.39%。结论:该测定方法快速、准确,可为水栀子的质量分析方法提供参考。Objective To establish an dual - wavelength HPLC method for the simultaneous determination of two compounds in the fruits of Gardenia jasminoides var. radicans. Methods The chromatographic separation was performed on a Cosmosil - 5C18 - AR - Ⅱ column （250mm ×4.6mm,5μm） with a gradient elution of methanol and 0. 1% acetic acid solution, at a flow rate of 1.0mL/min, and detected at both 238nm （for geniposide, 1 ） and 440nm （for crocin - I , 2）. Results The two compounds were separated with satisfaction, with linearity ranges between 50. 16 -501.6μg/mL （r =0. 9999, 1 ） and 10. 13 -101.3μg/mL （r =0. 9997, 2）, and average recovery rates to be 97. 77% and 97. 39%, respectively. Conclusion The method is accurate and selective, which could be applied for the quality analysis of the fruits of Gardenia genus.

关 键 词：水栀子 HPLC 栀子苷 西红花苷-Ⅰ 

分 类 号：R285[医药卫生—中药学]