人工过氧化物酶研究技术手段现状分析  

The Technological Means on Artificial Peroxidase

作　　者：韩明睿 王冬瑞 张牛冰 金洳西 蔡雨阳 宁艳娜 李迪[1] 肖保林[1] 洪军[1] 

机构地区：[1]河南大学生命科学学院,河南开封475000

出　　处：《山东化工》2017年第19期195-197,共3页Shandong Chemical Industry

摘　　要：天然过氧化物酶是一种以铁卟啉血红素(heme)为辅基,参与生命体内生理代谢的主要酶类之一。因为天然酶具有极易变性失活,提取相对困难,价格比较昂贵的缺点。故通过人工的技术手段制取价格低廉,性能稳定的人工酶。而对于人工过氧化酶的研究,技术手段就显得尤为重要。本文通过分析不同的技术手段:紫外分光光度计时间扫描研究过氧化物酶活性,圆二色光谱法研究蛋白质二级结构,紫外分光光度计光谱扫描研究蛋白质广谱结构,电化学法研究电子传递效应描述了自组装人工过氧化物酶构建及应用,分析人工过氧化物酶研究技术手段现状。The natural peroxidase is an enzyme,which has iron porphyrin heme auxiliary basis.It plays an important role on physiological metabolism in life.Natural enzymes have difficulty in extracting,extracting inactivation and costly defects.On the contrary,it is easy to extract artificial enzymes.It is cheap and stable performance.So,it is important for the research of artificial peroxidase to analyze the technological means.The article is to analyze technological means on artificial enzymes.It is divided into five parts,ultraviolet-visible spectrophotometer to calculate enzymatic activity,circular dichroism to research secondary protein structure,ultraviolet-visible spectrophotometer to analyze protein broad-spectrum structure and electrochemistry to research how to extract artificial peroxidase and put into use.

关 键 词：人工过氧化物酶 紫外可见光分光光度法 圆二色光谱法 电化学法 自杀性失活研究 

分 类 号：Q554.6[生物学—生物化学]