HPLC法同时测定干髓糊剂中丁香酚和麝香草酚的含量  

Simultaneous Determination of Eugenol and Thymol in Mummification Pastes by HPLC

作　　者：曹伟宇 贺文娟 雷丸 王晓娟 CAO Weiyu HE Wenjuan LEI Wan WANG Xiaojuan(State Key Lab of Military Stomatology/National Clinical Research Center for Oral Diseases/Shaanxi Engineering Research Center for Dental Materials and Advanced Man- ufacture/Dept, of Pharmacy, Stomatology Hospital of Fourth Military Medical University, Xi＇an 710032, China)

机构地区：[1]军事口腔医学国家重点实验室/国家口腔疾病临床医学研究中心/陕西省口腔生物工程技术研究中心/第四军医大学口腔医院药剂科,西安710032

出　　处：《中国药房》2017年第30期4268-4271,共4页China Pharmacy

基　　金：军队医疗机构制剂标准提高研究项目(No.13ZJZ08)

摘　　要：目的:建立同时测定干髓糊剂中丁香酚和麝香草酚含量的方法。方法:采用高效液相色谱法。色谱柱为Wonda Cract C_(18),流动相为甲醇-0.2%磷酸溶液(梯度洗脱),流速为1.0 mL/min,检测波长为274 nm,柱温为25℃,进样量为10μL。结果:丁香酚和麝香草酚检测质量浓度线性范围分别为0.02~0.19 mg/mL(r=0.999 7)、0.03~0.30 mg/mL(r=0.999 6);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为95.85%~99.84%(RSD=1.11%,n=9)、95.81%~100.91%(RSD=1.59%,n=9)。结论:该方法操作简便、结果准确,可用于干髓糊剂中丁香酚和麝香草酚含量的同时测定。OBJECTIVE：To establish a method for simultaneous determination of eugenol and thymol in mummification pastes. METHODS：HPLC method was adopted. The determination was performed on Wonda Cract C_（18） column with mobile phase consisted of methanol-0.2% phosphoric acid（gradient elution）at the flow rate of 1.0 mL/min. The detection wavelength was 274 nm,and column temperature was set at 25 ℃. The sample size was 10 μL. RESULTS：The linear ranges of eugenol and thymol were 0.02-0.19 mg/mL（r=0.999 7）and 0.03-0.30 mg/mL（r=0.999 6）. RSDs of precision,stability and reproducibility tests were all lower than 2.0%. The recoveries were 95.85%-99.84%（RSD=1.11%,n=9）and 95.81%-100.91%（RSD=1.59%,n=9）. CONCLUSIONS：The method is simple and accurate,and can be applied to simultaneous determination of eugenol and thymol in mummification pastes.

关 键 词：高效液相色谱法 干髓糊剂 丁香酚 麝香草酚 含量测定 

分 类 号：R927.2[医药卫生—药学]