芪明颗粒对糖尿病肾病大鼠肾组织WT1、AngⅡ、ET-1的影响  

Effects of Qiming granule on WT1,Ang Ⅱ and ET-1 in renal tissue of diabetic nephropathy rats

作　　者：王军媛[1] 赵建红[1] 刘颖[1] 张军[1] 李振[1] 臧乐红 

机构地区：[1]唐山市中医医院内分泌科,河北唐山063000

出　　处：《吉林中医药》2017年第10期1025-1028,共4页Jilin Journal of Chinese Medicine

基　　金：2014年度河北省中医药管理局科学技术课题(2014101)

摘　　要：目的观察芪明颗粒对糖尿病肾病模型大鼠肾组织中WT1、AngⅡ、ET-1表达的影响,研究其对糖尿病肾病的作用机制。方法选用SPF级SD大鼠50只,除空白组外各组给予链脲佐菌素单次腹腔注射造模,随机分为空白组、模型组、低剂量组、高剂量组、缬沙坦组。8周后分别用Westernblot法、ELISA法检测肾组织中WT1、ET-1、AngⅡ的表达。结果模型组肾组织WT1、AngⅡ、ET-1明显高于空白对照组(P<0.05);与模型组相比,低剂量组、高剂量组、缬沙坦组肾脏组织WT1、AngⅡ、ET-1均显著降低(P<0.05);高剂量组、缬沙坦组肾组织WT1、AngⅡ、ET-1明显低于中药低剂量组(P<0.05),中药高剂量组、缬沙坦组相比差异无统计学意义(P>0.05)。结论芪明颗粒可以有效的降低糖尿病肾病大鼠肾组织WT1、AngⅡ、ET-1的表达,以起到保护肾脏的作用。高剂量芪明颗粒较低剂量对肾脏有更好的保护作用。早期采用芪明颗粒对糖尿病进行干预,可以延缓肾损害的进展。为临床广泛应用芪明颗粒提供实验基础和理论依据。Objective To observe the effect of Qiming granule on the expression of WT1,Ang Ⅱ and ET-1 in renal tissue of DN rats,and to study the mechanism of DN.Methods 50 SD grade SPF rats were selected and randomly divided into blank group,model group,low dose group,high dose group and valsartan group.The rats were randomly divided into blank group,model group,low dose group,high dose group and valsartan group.After 8 weeks,the expression of WT1,ET-1 and Ang Ⅱ in renal tissues were detected by Westernblot and ELISA methods respectively.Results The renal tissue of model group WT1,Ang Ⅱ,ET-1 was significantly higher than control group（P0.05）; compared with the model group,low dose group,high dose group,valsartan group and kidney tissue WT1,Ang Ⅱ and ET-1 were significantly decreased（P0.05）; high dose group,valsartan group of renal WT1,Ang Ⅱ,ET-1,the low dose group was significantly lower than that of traditional Chinese medicine（P0.05）,high dose of Chinese medicine group,valsartan group showed no significant difference（P0.05）.Conclusion Qiming granule can effectively reduce the expression of WT1,Ang and ET-1 in renal tissue of DN rats,which can protect the kidney.High dose of Qiming granule has better protective effect on kidney.In the early stage,it can delay the progression of renal damage by using Qiming granule to interfere with diabetes.To provide experimental basis and theoretical basis for the clinical application of Qiming granule.

关 键 词：糖尿病肾病 血管紧张素Ⅱ 内皮素-1 肾母细胞肿瘤1 芪明颗粒 

分 类 号：R259[医药卫生—中西医结合]