42个苜蓿品种间亲缘关系的RAPD分析  

Analysis of Genetic Relationship of 42 Alfalfa Varieties by RAPD

作　　者：孙卉 朴政玉[2] 曲柏宏[2] 孙祎龙[3] SUN Hui PIAO Zhengyu QU Baihong SUN Yilong(Jilin Engineering Vocational College, Siping 136001 Agricultural College of Yanbian University, Yanfi 133002 Jilin Academy of Agricultural Sciences, Changchun 130033, China)

机构地区：[1]吉林工程职业学院,吉林四平136001 [2]延边大学农学院,吉林延吉133002 [3]吉林省农业科学院,长春130033

出　　处：《东北农业科学》2017年第5期30-35,共6页Journal of Northeast Agricultural Sciences

基　　金：吉林省自然科学基金(20150101111JC)

摘　　要：应用RAPD标记,对42个不同苜蓿品种的亲缘关系进行研究。采用CTAB改良法可快速提取到较高质量苜蓿基因组总DNA用于RAPD扩增,并从RAPD反应中筛选出19个10碱基随机引物,总共扩增出220条带,其中138条呈多态性,占62.7%,82条为单态性带,占37.3%;利用MEGA2.1软件计算42个苜蓿品种遗传距离,进行UPGMA聚类分析,遗传距离在0.174~0.500之间,说明供试苜蓿品种间遗传距离较大,亲缘关系较远。遗传距离为0.435时,42个苜蓿品种可分为五类,不同苜蓿品种间的亲缘关系有较大的差异,表明RAPD标记可作为苜蓿品种亲缘关系判定的依据。这为苜蓿品种遗传改良、杂交亲本选配提供可靠的科学依据。The genetic relationships of 42 alfalfa varieties were studied using RAPD analysis. The results showedthat it is helpful to improve the genomic DNA quality used for RAPD amplification by using modified CTAB meth-od. 19 random primers composed of 10 bases were selected from the RAPD markers, and there were 138 polymor-phism fragments in the 220 amplified fragment found, accounting for 62.7%, 82 monomorphism fragments, account-ing for 37.3%. MEGA2.1 software was used for calculating genetic distance of 42 alfalfa varieties. There were a dis-tant relationship among them because the range of genetic distance was 0.174-0.5000. 42 alfalfa varieties can be di-vided into 5 groups when genetic distance was 0.435. RAPD markers can be used as the basis for classification of al-falfa genetic relationship, because the genetic relationship of different alfalfa varieties have bigger difference. Thestudy was done to provide reliable scientific basis for improvement and hybrid parents selection of alfalfa variety.

关 键 词：苜蓿 RAPD 亲缘关系 遗传距离 

分 类 号：S54[农业科学—作物学]