外周血DNA甲基化谱与吸烟的关系  

The relationship between DNA methylation profile of peripheral blood and smoking

作　　者：毕钢[1] 曹禹[1] 胡伟[1] 沈玮玮 周怀谷[1] 刘赟 

机构地区：[1]上海市公安局物证鉴定中心,上海200083 [2]上海市公安局松江分局刑事科学技术研究所,上海201600 [3]复旦大学基础医学院生物化学与分子生物学系,上海200032

出　　处：《中国法医学杂志》2017年第5期508-511,共4页Chinese Journal of Forensic Medicine

基　　金：国家自然科学基金面上项目(31471212)

摘　　要：目的通过比较吸烟与不吸烟个体外周血DNA甲基化谱的差异,评估DNA甲基化在区分吸烟与不吸烟人群中的应用价值。方法根据下载于NIH的GEO公共数据库的人类全基因组DNA甲基化数据,运用Student’s T检验和聚类分析的方法评估外周血特定CpG位点DNA甲基化程度在吸烟与不吸烟人群中的差异。结果特定Cp G位点上,非吸烟人群与吸烟人群的DNA甲基化有显著区别。结论检测人外周血的甲基化情况可以区分吸烟和非吸烟人群。Objective To evaluate the potential application of separating smoking individuals from non-smoking ones by DNA methylation profiles from peripheral blood. Methods Human genome-wide DNA methylation data were downloaded from NIH GEOdata base. DNA methylation values from certain Cp G sites were used to evaluate their significance between smokers and non-smokers by Student＇s T Test, as well as the clustering analysis. Results There are significant DNA methylation between smokers and non-smokers for certain Cp Gs. Conclusion Detection of methylation status from human peripheral blood can distinguish smokers from non-smokers.

关 键 词：法医物证学 甲基化 个体识别 吸烟人群 

分 类 号：D919.4[医药卫生—法医学]