不同方法确定动情周期及动情期生殖器官结构  

Different methods to determine estrus cycle and the structure of reproductive organs

作　　者：覃海章 肖胜军 华英杰 卢德杰 米超 赵玮 章一帆 任亚萍[5] 

机构地区：[1]桂林医学院临床医学院,桂林541004 [2]桂林医学院第二附属医院病理科,桂林541100 [3]桂林医学院研究生学院,桂林541004 [4]桂林医学院公共卫生学院,桂林541004 [5]桂林医学院组织胚胎学教研室,桂林541004

出　　处：《安徽医科大学学报》2017年第11期1650-1653,共4页Acta Universitatis Medicinalis Anhui

基　　金：广西自然科学基金项目(编号:2016GXNSFAA380319);桂林市科学研究与技术开发计划项目(编号:20130120-8)

摘　　要：目的为确定最佳合笼时间,对两种常见鉴定小鼠动情周期方法进行比较,并确定处于动情期的子宫内膜和输卵管的形态结构,为动物生殖发育及胚胎研究提供基础技术指导。方法对43只昆明小鼠分别应用阴道脱落细胞检查法、宫颈黏液结晶法,确认小鼠动情周期,与子宫、输卵管和卵巢做成石蜡切片,HE染色后镜下观察的结果进行统计分析比较,同时对处于动情期的小鼠子宫内膜和输卵管进行HE染色。结果阴道脱落细胞检查法所确定小鼠动情周期与子宫镜检所见确定的动情周期中,在动情期的判定上吻合率较高;小鼠的阴道脱落细胞在动情周期各个时期有不同的形态特点,处于动情期的小鼠的子宫内膜和输卵管的形态与处于非动情期的小鼠有明显变化。结论阴道脱落细胞染色检查确定动情期比宫颈黏液结晶法更为可靠;小鼠激素水平的变化使阴道上皮细胞发生周期性变化,动情期小鼠输卵管和子宫内膜发生相应改变分别为受精和着床做准备。Objective To provide basic technical guidance for reproduction and development of animals and em- bryo research, two common methods of identifying estrous cycle in mice are compared to determine optimal mating time, and morphological structure of edometrium and oviduct in estrus is identifed. Methods 43 Kunming female mice were tested by vaginal smear observation and cervical mucus crystallization to determine estrous cycle. The re- sults of microscopic observation of uterus, oviducts and ovaries, which were paraffin-embedded and then stained with hematoxylin-eosin staining, were statistically analyzed and compared. While endometriums and oviducts of the mice in estrus were stained with HE. Results Vaginal smear observation had a higher coincidence rate in the judg- ment of estrus with microscopic examination of uterus. Cal characteristics at different stages of estrous cycle. The vaginal exfoliated cells of mice had different morphologi- The endometrium and oviduct of the mice in estrus changed significantly in morphology with those in non-estrus. Conclusion Vaginal smear observation is more reliable than cervical mucus crystallization todetermine estrus. The changes of hormone level in mice made the morphologyof va- ginal epithelial cells change cyclically, and the corresponding changes in the oviduct and endometrium are prepared for fertilization and implantation.

关 键 词：动情周期 阴道脱落细胞学 输卵管 子宫内膜 小鼠 

分 类 号：R339.2[医药卫生—人体生理学]