基质金属蛋白酶-9基因多态性与2型糖尿病视网膜病变的相关性研究  

A Study of the Association between Polymorphisms of the MMP-9 Gene and type 2 Diabetic Retinopathy

作　　者：杨洁[1] 马英慧[1] 董微丽[1] 崔秀成[1] 石晶[1] 贾淑君[1] 

机构地区：[1]承德医学院附属医院眼科,河北承德067000

出　　处：《河北医学》2017年第10期1663-1667,共5页Hebei Medicine

摘　　要：目的:研究基质金属蛋白酶9(MMP-9)-1562C/T基因多态性与中国北方汉族人群2型糖尿病视网膜病变的关系。方法:采用限制性片段长度多态性聚合酶链反应(PCR-RFLP)方法检测了180名健康对照者和400例2型糖尿病患者(其中单纯2型糖尿病患者180例、糖尿病视网膜病变患者220例)的MMP-9基因型,比较各组之间基因型及等位基因频率的差别。结果:MMP-9-1562C/T正常对照组,糖尿病组,糖尿病视网膜病变组三组C、T等位基因频率分别为8.3%,91.7%;7.8%,92.2%;91.4%,8.6%,三组等位基因频率间差异有统计学意义(χ~2=790.09,P=0.000)。三组C/C,C/T,T/T基因型频率分别为0%,16.7%,83.3%;2.2%,11.1%,86.7%;84.1%,14.5%,1.4%,三组基因型频率之间差异无统计学意义(χ2=470.16,P=0.000),其中正常对照组与糖尿病视网膜病变组之间差异有统计学意义(P<0.01);正常对照组与糖尿病组之间差异无统计学意义(P>0.01);糖尿病组与糖尿病视网膜病变组之间差异无统计学意义(P<0.01)。与T等位基因型相比,C等位基因可能会增加2型糖尿病视网膜病变的发病风险。结论:MMP-9基因-1562C/T多态性可能与2型糖尿病视网膜病变的发生有关,T等位基因可能是2型糖尿病视网膜病变的保护基因。Objective： To study of the association between polymorphism of the MMP-9 gene（-1562 C/T） and type 2 diabetic retinopathy. Methods： MMP-9-1562 C/T SNP was genotyped by polymerase chain reaction-restrictive fragment length polymorphism（ PCR-RFLP）,analysis in 180 healthy persons as control and 400 patients with type 2 diabetes including 180 complication-free cases and 220 with diabetic retinopathy（ DR）. Results： Association between the MMP-9-1562 C/T SNP in promoter region and diabetic retinopathy： the C and T allele frequencies in Control,DM,DR groups are 8.3%,91.7%; 7.8%,92.2%; 91.4%,8.6% respectively. And the results have a significant deviation（ χ~2= 790.09,P = 0.000）. The C/C,C/T,T/T genotypic frequencies of cases in the three groups are 0%,16.7%,83.3%; 2.2%,11.1%,86.7%; 84.1%,14.5%,1.4%.There＇s a significant deviation of C and T allele frequency of MMP-9-1562 C/T between the Control group、the DM group and DR group（ χ~2= 470.16,P = 0.000）.There＇s a significant deviation of C/C,C/T,T/T genotypic frequencies between the Control group and DM group（ P 〈0.01）,or the DM group and DR group（ P〈0.01）,while there is no significant deviation between the Control group and DM group（ P〉0.01）.Compared with the T allele,the C allele may increase the onset risk of diabetic retinopathy. Conclusion： The MMP-9-1562 C/T SNP may play a role in the genetic susceptibility to the invasion of diabetic retinopathy. T allele is a protective gene for diabetic retinopathy.

关 键 词：糖尿病视网膜病变 基质金属蛋白酶-9 基因 单核苷酸多态性 

分 类 号：R587.2[医药卫生—内分泌]