白杨派无性系苗期耐盐性综合评价及筛选  

Comprehensive evaluation and screening of salt tolerance for Leuce clones at nursery stage

作　　者：杨传宝 孙超 李善文[3] 姚俊修[3] 刘敬国 矫兴杰 

机构地区：[1]国家林业局竹子研究开发中心 [2]山东省烟台市林木种苗站 [3]山东省林业科学研究院山东省林木遗传改良重点实验室 [4]山东省沂南县林业局 [5]山东省牟平区林业局

出　　处：《北京林业大学学报》2017年第10期24-32,共9页Journal of Beijing Forestry University

基　　金：山东省农业良种工程项目(2012213);山东省重点研发计划项目(2017GNC11115)

摘　　要：以10个白杨派无性系为试材进行盆栽试验,通过对比各无性系的盆栽苗在不同质量分数(0、0.1%、0.3%、0.5%和0.7%)NaCl单盐胁迫条件下生长和生理代谢等参数的变化,对白杨派无性系进行了耐盐性能分析与评价。结果表明:高盐胁迫明显抑制了10个无性系苗高和地径的增长率(P<0.05),无性系0057表现出较强的速生性和高存活率;低盐胁迫对无性系生长和细胞膜系统的影响不明显,但能诱导细胞内抗氧化酶(SOD和POD)活性和可溶性蛋白含量的上升;随着土壤盐浓度的增加以及盐处理时间的延长,保护酶活性、可溶性蛋白含量、细胞膜透性和MDA含量与对照相比均有显著提高。无性系的SOD与POD、可溶性蛋白之间的耐盐系数呈显著正相关关系,POD与MDA之间的耐盐系数呈显著负相关,表明无性系可以通过积累大量可溶性蛋白,增强抗氧化酶活性减少MDA的积累,提高自身耐盐能力。利用主成分分析和隶属函数法对10个无性系的耐盐性进行综合评价,初步认为无性系0057、422和XJY具有显著耐盐性和速生性,可作为盐碱地造林或抗逆育种的重要试验材料应用。Ten white poplar clones were used as the materials in this study to analyse the changes of growth and physiological indexes under different salt stresses（ 0, 0. 1%, 0. 3%, 0. 5% and 0. 7% NaCl concentration）. The results showed that the height growth rate and the basal diameter growth rate of ten white poplar clones were inhibited apparently under high salt stress（ P 0. 05）. Clone 0057 showed fastgrowing performance and high survival rate. The effects of low salt stress on the growth and cell membrane system of clones were not obvious,but it could induce the increase of antioxidant enzyme（ SOD and POD） activity and soluble protein content. With the increase of NaCl concentration and the extension of salt treatment time,the protective enzyme activity,soluble protein content,cell membrane permeability and MDA content were significantly increased compared with the control. The salt tolerancecoefficients of POD and soluble protein in ten clones were positively correlated with SOD,and the salt tolerance coefficient between POD and MDA was negatively correlated. The results showed that clones could reduce the accumulation of MDA and improve their salt tolerance by accumulating a large amount of soluble protein and enhancing the activity of antioxidant enzymes. Based on the principal component analysis and membership function analysis, the salt tolerance of ten clones was evaluated comprehensively. It is preliminarily considered that clones 0057,422 and XJY have significant salt tolerance and fast-growing performance,which could be used as the important materials for afforestation in saline soil and breeding resource in resistance research.

关 键 词：白杨派 NACL胁迫 生理指标 耐盐性 

分 类 号：S722.33[农业科学—林木遗传育种] S792.117[农业科学—林学]