NaHCO_3胁迫对转TaLEA基因山新杨生长及光合、叶绿素荧光特性的影响  

Effects of NaHCO_3 stress on growth,photosynthesis and chlorophyll fluorescence characteristics in Populus davidiana × P. bolleana overexpressed TaLEA

作　　者：孙延爽 邢宝月 杨光 刘桂丰 

机构地区：[1]东北林业大学林学院林木遗传育种国家重点实验室

出　　处：《北京林业大学学报》2017年第10期33-41,共9页Journal of Beijing Forestry University

基　　金："863"国家高技术研究发展计划项目(2011AA100201)

摘　　要：为了筛选耐苏打盐渍土的转基因杨树优良株系,以苏打盐渍土的主要成分NaHCO_3胁迫处理转Ta LEA基因的山新杨各株系与对照(NT),测定不同株系的苗高和地径等生长性状,测定净光合速率(Pn)、胞间CO2浓度(Ci)、气孔导度(Gs)、蒸腾速率(Tr)等光合参数以及最大光化学效率(Fv/Fm)、光化学猝灭系数(q P)、非光化学猝灭系数(NPQ)、实际光化学电子产量(ΦPSII)等叶绿素荧光动力学参数,利用染色法分别比较各株系的超氧离子和过氧化氢的累积情况,进而综合评价不同株系对NaHCO_3胁迫的响应。结果表明,NaHCO_3胁迫处理前、后各株系的苗高和地径生长量发生明显分化,由处理前的差异不显著,到处理20 d后的显著,转基因株系的苗高、地径普遍较对照高,表现突出的是转基因株系SL2号,其平均苗高和地径分别较对照提高了17.4%、15.7%;对照的盐害指数是转基因株系的3.3倍,并且转基因株系的平均存活率是对照的2.8倍。各转基因株系的光合参数和荧光参数也较对照增强,Pn、Tr在胁迫20 d后转基因株系较对照分别高了237.38%、649.02%,Gs、Ci在胁迫15 d时转基因株系较对照分别高了119.05%、24.56%,其中SL2号转基因株系较对照分别提高150%、25.81%。各转基因株系的Fv/Fm平均为0.68,对照只有0.45,前者的q P和ΦPSII只略高于后者,后者的NPQ却是前者的6倍。转基因株系的超氧离子和过氧化氢累积量均少于对照。在相同的碱胁迫环境下,转基因山新杨,尤其是SL2,能保持较快的生长量、较强的光合能力和较低的ROS水平,初步选择其为耐苏打盐渍土的杨树转基因优良株系。In order to find the transgenic poplar which can tolerate soda-saline soil,the lines overexpressed Ta LEA gene and the control（ NT） were treated with Na HCO3,the main components of soda-saline soil.And to evaluate salt-tolerant properties,we measured some parameters of different lines,including height and ground diameter,net photosynthetic rate（ Pn）,intercellular CO2 concentration（ Ci）,stomatal conductance（ Gs）,transpiration rate（ Tr）,maximal photochemical efficiency（ Fv/Fm）,photochemical quenching coefficient（ q P）, non-photochemical quenching coefficient（ NPQ）,and the actual photochemical electron yield（ ΦPSII）. Moreover,the accumulation of superoxide anion and hydrogen peroxide in each line was compared by staining method. The results showed that obvious changes had taken place in height and ground diameter among different lines after Na HCO3 stress. There was no significant difference before treatment,but after treating for 20 days,significant differences happened.Compared with control,all transgenic lines increased in height and ground diameter,especially the SL2transgenic line,whose average height and ground diameter were increased by 17. 4% and 15. 7%,respectively. Furthermore,the salt damage index of wild type was 3. 3 times of transgenic lines,and the survival rate of transgenic lines was 2. 8 times of wild type. The photosynthetic parameters and fluorescence parameters of the transgenic lines after 20 days stress were also higher than wild type,Pn and Trwere increased by 237. 38% and 649. 02%,respectively compared with the wild type. Compared with control,Gsand Ciof transgenic lines were increased by 119. 05% and 24. 56% under 15 days stress,and Gs,Ciof SL2 transgenic line were increased by 150% and 25. 81%. Besides,the average Fv/Fmof each transgenic line was 0. 68,the wild type was 0. 45. The q P and PSII of transgenic plants were only slightly higher than that of the wild type,but the NPQ of wild type was 6 times of wild type.The accumulation of superoxide anion a

关 键 词：转基因 NAHCO3胁迫 生长 光合作用 叶绿素荧光 TaLEA 

分 类 号：S722.36[农业科学—林木遗传育种]