A stage-specific protein factor binding to a CACCC motif in both human β-globin gene promoter and 5'-HS2region  

作　　者：SUN TONG- YADI CHEN YULONG HU CHANGHONGDAI RUOLAN QIAN.(Shanghai Institute of Cell Biology, Academia Sinica,Shanghai 200031- China） 

出　　处：《Cell Research》1994年第2期135-143,共9页细胞研究（英文版）

摘　　要：The DNasel hypersensitive site 2 (HS2) of human β-globin locus control region (LCR) is required fOr the high level expression of human d-globin genes. In the present study, a stage-specific protein factor (LPF-β) was identified in the nuclear extract prepared from mouse fetal liver at d 18 of gestation, which could bind to the HS2 region of humanβ-globin LCRt We also found that the shift band of LPF-βfactor could be competed by humanβ-globin promoter. However, it couldn’t be competed by human E-globin promoter or by human Aβ-globin promoter. Furthermore, our data demonstrated that the binding-sequence of LPF-d factor is 5’CACACCCTA 3’,which is located at the HS2 region ofβ-LCR (from -10845 to -10853 bp) and humanβ-globin promoter (from -92 to -84 bp). We speculated that these regions containing the CACCC box in both the humallβ-globin promoter and HS2 might function as stage selector elements in the regulation of humanβd-globin switching and the LPF-βfactor might be a stage-specific protein factor involved in the regulation of humanβ-globin gene expression.

关 键 词：β-globin promoter HS2 of β-globin LCR trans-acting factor 

分 类 号：Q344.5[生物学—遗传学] Q132.7