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作 者:WEI ZHIMING ZHIHONG XU JIANQIU HUANG NONG XU MINREN HUANG.(National laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology, Academia Sinica, 300Fenglin Road. Shanghai 200032, China)(Forest Tree Genetics and Breeding Laboratory
出 处:《Cell Research》1994年第2期183-189,共7页细胞研究(英文版)
摘 要:Morus alba(white mulberry) mesophyll protoplasts were isolated from leaves of 30-45 day old sterile shoots,with protoplast yields of 2.5 x 107 g-1/F.W. after purification. The protoplasts were cultured in a modified K8P liquid medium containing 0.2 mg/L 2,4-D(2,4- Dichlorophe-noxy acetic acid), 1 mg/L NAA(Naphthyl acetic acid) and 0.5 mg/L BA(6-benzylaminopurine). A low plating density (5 x 104/ml) proved to be favourable to the division of protoplast-derived cells. The first divisioll occurred 4 days after culture, and the division frequency reached 24% at 10 days. A number of cell colonies and microcalli formed in 6 weeks. The microcalli were transferred onto MSB medium with 0.5 mg/L NAA and 0.5 mg/L BA for further proliferation. Shoot formation was initiated when the calli of 3-4 mm in size were transferred onto MSB differentiation medium with 0.1 mg/L NAA and 1 mg/L BA. The frequency of shoot formation was 35%. The shoots of 4-5 cm in height were excised from the callus and rooted on half strength MS medium with 0.5 mg/L IBA and 0.1 mg/L BA. After transplantation into pots, the regenerated plants grew vigorously in the phytotron.
关 键 词:Morus alba L. white mulberry protoplast culture plant regeneration
分 类 号:Q943.1[生物学—植物学] S888.32[农业科学—特种经济动物饲养]
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