超声辐照比格犬左室心肌应变的在体实验研究  

Effects of different ultrasound power application on left ventricular myocardium 2-dimension strain in in vivo open-chest beagle canines

作　　者：李文华 尹立雪 张红梅 杨树[2] 陆景 苗俊旺[3] 林嶷 黄宇 丁戈琦 邓燕 

机构地区：[1]四川省医学科学院四川省人民医院超声医学研究所超声心脏电生理学与生物力学四川省重点实验室,成都610072 [2]四川省医学科学院四川省人民医院神经内科,成都610072 [3]山西医学科学院山西大医院超声科

出　　处：《中华超声影像学杂志》2017年第10期899-905,共7页Chinese Journal of Ultrasonography

基　　金：国家自然科学基金项目（81671852）;四川省科技厅项目（2016TD0017,2017TD0015）

摘　　要：目的探讨不同超声波声能辐照及微泡造影剂干预开胸比格犬在体左心室心肌二维应变变化。方法12只健康比格犬开胸模型，随机分为A、B两组（每组6只）。A组：诊断超声波声能约300mw，单纯诊断超声波辐照5min、注射微泡造影剂联合诊断超声波辐照5min和注射微泡造影剂后20min，分别采集左心室短轴二尖瓣水平、乳头肌水平、心尖水平，心尖四腔、心尖三腔、心尖二腔各3个完整心动周期实时灰阶二维图像；B组：超声波声能1W、2W、3W，分别辐照5min后采集左心室短轴二尖瓣水平、乳头肌水平、心尖水平，心尖四腔、心尖三腔、心尖二腔各3个完整心动周期实时灰阶二维图像。应用Syngo VVI工作站，分别获取节段和整体峰值周向应变（circumferential strain，CS）、纵向应变（10ngitudinal strain，LS）、径向应变（radial strain，RS）的二维力学参数。结果A组：与基础状态相比，诊断超声波辐照、微泡造影剂联合诊断超声波辐照5min、微泡造影剂后20min整体峰值CS及LS增大，差异均有统计学意义（P〈0．05）；整体峰值RS无明显变化，差异无统计学意义（P〉0．05）。B组：与基础状态相比，超声波声能1W辐照整体峰值CS及LS显著增大（均P〈0．05），整体峰值RS呈增大趋势，但差异无统计学意义（P〉0．05）；超声波声能2W辐照整体峰值CS、LS、RS均呈降低趋势，但差异无统计学意义（均P〉0．05）；超声波声能3w辐照整体峰值CS、整体峰值LS均降低，差异均有统计学意义（均P〈0．05），整体峰值RS呈降低趋势，差异无统计学意义（P〉0．05）。结论诊断超声波辐照、微泡造影剂联合诊断超声波辐照、微泡造影剂后20min、超声声能1W辐照诱导心肌CS、LS增大产生正性肌力效应，超声声能3W辐照诱导心肌CS、LS降低产生负性肌力效应，而同一干预因素对心肌RS无明显影响。Objective To evaluate the left ventricular （LV） myocardial mechanical changes with contrast agent and different power irradiation in open-chest beagle canines. Methods The anesthetized open-chest beagle canines were assigned into two groups randomly：group A （ n = 6） for baseline, diagnostic ultrasound power irradiation （300 mW） 5 min, combined with contrast agent irradiation 5 rain and contrast agent 20 min conditions;group B （ n = 6） for baseline and intensity ultrasound irradiation （1 W, 2 W and 3 W, 5 min respectively） conditions. The peak circumferential strain （CS）, longitudinal strain （LS） and radial strain （RS） of LV myocardial wall were measured and analyzed using a dedicated Syngo velocity vector imaging （VVI） method. Results In group A, the peak CS and LS were increased significantly on diagnostic power irradiation,contrast agent irradiation 5 rain and contrast agent 20 min （all P 〈0.05, respectively） compared with baseline condition; there was no significant diHerence of the peak RS on diagnostic power irradiation, contrast agent irradiation 5 min and contrast 20 min compared with baseline condition （ P 〉 0. 05）. In group I3, the peak CS and LS were higher on power i W than on baseline condition （ P 〈0. 05）, the peak RS was increased gradually on power 1 W than on baseline condition （ P 〉0.05） ; the peak CS, LS, RS were lower on power 2 W than on baseline condition （ P 〉0.05） ; the peak of CS, LS were lower on power 3 W than on baseline condition （P 〈 0.05）;the peak of RS was decreased gradually without significant changes between power 3 W on baseline condition （ P 〉0.05）. Conclusions These findings indicate potential positive inotropic effects with diagnostic ultrasound power irradiation 5 min, combined with contrast agent irradiation 5 rain, contrast agent 20 min conditions, power 1W and negative inotropic effects with power 3 W on LV myocardial CS, LS deformation. LV myofiber deformation reactions appear no significant

关 键 词：超声处理 微气泡 心室功能 左 应变 

分 类 号：R540.45[医药卫生—心血管疾病]