Identification of γ-aminobutyric acid transporter (GAT1)on the rat sperm  

作　　者：HUJINGHUA XIAOBINGHE 等 

机构地区：[1]ShanghaiInstituteofCellBiology,ChineseAcademyofSciences,Shanghai200031 [2]ShanghaiInstituteofCellBiology

出　　处：《Cell Research》2000年第1期51-58,共8页细胞研究（英文版）

基　　金：grants of National "Pan Deng" program; LMCBand National Natural Science FOundation!No: 39770776

摘　　要：Some recent studies indicated that GABAergic system is involved in mammalian sperm acrosome reaction (AR), but direct evidence pertaining to the expression of gat1 in mammalian sperm is not yet demonstrated. In this study, we evaluated the presence of 67kDa GAT1 protein and mRNA in rat testis by Western blotting and reverse transcription-polymerase chain reaction. Meanwhile, immunohistochemical and immunofluorescent analyses also identified GAT1 protein on the elongated spermatid and sperm. These results indicated that rat testis is a novel site of gat1 expression. Further studies should be taken to explore the role of GAT1 protein on sperm acrosome reaction.

关 键 词：GAT1 rat testis SPERM expression. 

分 类 号：Q954[生物学—动物学]