miR-146b对人单核细胞株THP-1细胞COX2表达的影响及可能机制  

Lentiviral-mediated microRNA-146b regulates COX2 expression by p38MAPK

作　　者：杨铭[1] 陆林[2] 吴方[1] YANG Ming LU Lin WU Fang(Department of Geriatrics Department of Cardiology, Affiliated Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China)

机构地区：[1]上海交通大学医学院附属瑞金医院老年病科,上海市200025 [2]上海交通大学医学院附属瑞金医院心血管科,上海市200025

出　　处：《中国动脉硬化杂志》2017年第9期890-894,共5页Chinese Journal of Arteriosclerosis

基　　金：上海市自然科学基金(16ZR1430900)

摘　　要：目的探讨微小RNA-146b(miR-146b)对人单核细胞株(THP-1)细胞p38分裂素原活化蛋白激酶(p38MAPK)表达的影响。方法血管紧张素Ⅱ(AngⅡ)刺激THP-1建立细胞模型,慢病毒感染pre-miR-146b-3p或anti-miR-146b-3p进入THP-1细胞中,检测p38MAPK与环氧化酶2(COX2)表达的变化。转入p38MAPK siRNA,检测p38MAPK与COX2表达的变化。结果 AngⅡ刺激THP-1细胞p38MAPK与COX2表达升高,pre-miR-146b-3p可以放大这种增高效应,当转染p38MAPK siRNA后,能逆转pre-miR-146b-3p升高COX2的趋势(P<0.01)。结论在AngⅡ刺激的THP-1细胞中,miR-146b可能通过p38MAPK调控COX2表达水平,在心血管疾病的诊治中发挥重要作用。Aim To investigate the effect of microRNA-146b （miR-146b） on the expression of p38 Mitogen-Ac- tivated protein kinase（p38MAPK） in human acute monocytic leukemia cell line（THP-1）. Methods Cell model was established by angiotensin ]1 （ Ang Ⅱ ） stimulation. Pre-miR-146b-3p or anti-miR-146b-3p were transfected by lentivirus and the expression of p38MAPK and COX2 were detected, p38MAPK siRNA was transfected and the expression of p38MAPK and cyclooxygenase （COX2） in THP-1 cells were detected. Results The expression of p38MAPK and COX2 was increased in THP-1 cells stimulated by Ang Ⅱ and pre-miR-146b-3p could amplify the effect, p38MAPK siRNA decreased the ability of pre-miR-146b-3p to increase levels p38MAPK and COX2. The difference was statistically signifi- cant （P〈0.01）. Conclusion miR-146b may regulate COX2 expression by p38MAPK in Ang Ⅱ stimulated THP-1 cells, and may play an important role in the diagnosis and treatment of cardiovascular diseases.

关 键 词：miR-146b 人单核细胞株1 p38分裂素原活化蛋白激酶 环氧化酶2 慢病毒 

分 类 号：R4[医药卫生—临床医学]