抑郁症患者外周血单核细胞长链非编码RNA表达水平与抑郁症状严重程度的关系  

Correlation between the IncRNA expression level in peripheral blood mononuclear cell and the severity of major depressive disorder patients

作　　者：姚高峰[1] 张书友[2] 牛威[3] 何明俊 孔令明[1] 张理义[1] 

机构地区：[1]解放军第102医院全军心理疾病防治中心,常州213003 [2]解放军第102医院心身科,常州213003 [3]解放军第102医院康复科,常州213003

出　　处：《中华行为医学与脑科学杂志》2017年第10期883-888,共6页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：全军后勤应用基础研究重大项目（BWS14J027）

摘　　要：目的探讨抑郁症（major depressive disorder，MDD）患者外周血单核细胞中差异表达的长链非编码RNA（IncRNA）与抑郁症状严重程度之间的关系。方法连续纳入2014年5月至2015年2月门诊及住院收治的138例首发或3个月内未服药治疗的抑郁症患者作为研究对象，以实时荧光定量PCR技术检测患者外周血单核细胞中9种IncRNA（TCONS_12_00001212、NONHSAT102891、TCONS_00019174、ENST000005666208、NONHSAG045500、ENST00000591189、ENST00000617573、NONHSAT034045和NONHSAT142707）的表达水平，以24项汉密尔顿抑郁量表（HAMD-24）评估其抑郁严重程度。结果①TCONS_L2_00001212与绝望感因子呈显著正相关（r=0．370，P〈0.01）；TCONS_00019174与抑郁总分、迟缓因子及绝望感因子呈显著正相关（r=0．286，0．346，0．542，P〈0．01）；NONHSAT142707与抑郁总分及绝望感因子呈显著正相关（r=0．253，0．525，均P〈0．01）。②抑郁高分组和低分组患者TCONS_00019174和NONHSAT142707表达水平不同，差异有统计学意义（Z=3．238，2．254；P〈0．05）。③TCONS_00019174进入了抑郁总分的回归方程，解释其19．8％的变异，并对抑郁严重程度有一定的预测作用（AUC=0．833，P〈0．01），当TCONS_00019174=8．352CT值时，约登指数达最大值0．495，敏感度和特异度分别为64．9％和84．6％。结论TCONS_00019174对抑郁症患者严重程度有较好的正向预测效能。Objective To explore the eorrelation between the long non-coding RNA （lneRNA） expression in peripheral blood mononuelear eell （PBMC） and depressive symptoms in victims of major depression disorder （MDD）. Methods A total of 138 consecutive MDD patients who had not taken antidepressant drugs in the last 3 months or were first-episode patients from May 2014 to February 2015 were enrolled in the present study.The expressions of 9 MDD-assoeiated lneRNAs （TCONS_12_00001212,NONHSAT102891,TCONS_ 00019174,ENST00000566208, NONHSAG045500, ENST00000591189, ENST00000517573, NONHSAT034045 and NONHSAT142707） in PBMC were determined by real-time fluorescence quantitative PCR, and the severity of the depressive symptoms were evaluated by 24 item Hamilton depressive Seale （HAMD-24）. Results The expression level of TCONS L2 00001212 was significantly positively correlated with hopelessness symptoms （ r= 0.370,P〈0.01 ）, the expression level of TCONS_00019174 was significantly positively correlated with total depressive symptoms, retardation symptoms and hopelessness symptoms （ r= 0.286,0.346,0.542, all P〈0.01 ） and the expression level of NONHSAT142707 was significantly positively correlated with total depressive symptoms and hopelessness symptoms （ r= 0.253,0.525 ,P〈0.01 ） .The expression level of TCONS_00019174 and NONHSAT142707 in the higher scores subgroup was significantly lower than those in lower scores subgroup （ Z= 3.238,2.254, P〈 0.05 ）. When TCONS_00019174 entered into the regression equation with the total scores as the independent variable,it explained 19.8% of the variance of total scores.A ROC curve analysis revealed that TCONS_000191745 had moderate intensity prediction function on the severity of the depressive symptoms （AUC=0.833,P〈0.01）. When the cutoff value was 8.352 CT,Youden index was 0.495 in maximum with 88.24% sensitivity and 89.32% specificity. Conclusion TCONS_00019174 has a good positive prediction performance on symptom severity in MDD patients.

关 键 词：抑郁症 外周血单核细胞 长链非编码RNA 抑郁症状 实时荧光定量PCR 

分 类 号：R749.4[医药卫生—神经病学与精神病学]