Nuclear assembly of purified Crythecodinium cohnii chromosomes in cell-free extracts of Xenopus laevis eggs  

作　　者：LIU XIAO LING YAN SHEN ER JIE CHEN ZHONG HEZHAI(E-mail: [e-mail] Tel: [phone] Fax: [phone])(College of Life Sciences, Peking University, Beijing 100871,China) 

出　　处：《Cell Research》2000年第2期127-137,共11页细胞研究（英文版）

摘　　要：Incubation of dinoflagellate Crythecodinium cohnii chromosomes in cytoplasmic extracts of unfertilized Xenopus laevis eggs resulted in chromosomes decondensation and recondensation, nuclear envelope assembly, and nuclear reconstitution.Dinoflagellate Crythecodinium cohnii is a kind of primitive eukaryote which possesses numerous permanently condensed chromosomes and discontinuous double-layered nuclear membrane throughout the cell cycle. The assembled nuclei, being surrounded by a continuous double membrane containing nuclear pores and the uniformly dispersed chromatin fibers are morphologically distinguishable from that of Dinoflagellate Crythecodinium cohnii. However, incubation of dinoflagellate Crythecodinium cohnii chromosomes in the extracts from dinoflagellate Crythecodinillm cohnii cells does not induce nuclear reconstitution.

关 键 词：Dinoflagellate Crythecodinium cohnii CHROMOSOME nuclear reconstitution cell-free system Xenopus laevis egg extracts 

分 类 号：Q243[生物学—细胞生物学]