补肾健脾方对自然衰老小鼠生殖能力及体外受精中卵子质量的影响  

Effect of Bushen Jianpi Formula(补肾健脾方) on Reproductive Ability and Egg Quality of In Vitro Fertilization of Natural Aging Mice

作　　者：沈明洁[1] 齐聪[1] 

机构地区：[1]上海中医药大学附属曙光医院

出　　处：《中医杂志》2017年第21期1850-1855,共6页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81403424);上海市进一步加快中医药事业发展三年行动计划(ZY3-CCCX-3-3007;ZY3-RCPY-2-2009);上海市科学技术委员会科研计划(14ZR1442100)

摘　　要：目的观察补肾健脾方对自然衰老小鼠卵巢功能的影响及可能作用机制。方法将48只9月龄C57BL/6J雌性小鼠(自然衰老模型)随机分为模型组、阳性对照组和补肾健脾方高、低剂量组,每组12只,另选2~3月龄小鼠12只作为青年组。补肾健脾方低、高剂量组分别给予补肾健脾方0.51、2.55 g/(kg·d)灌胃,阳性对照组给予脱氢表雄酮片1.25 mg/(kg·d)灌胃,青年组及模型组给予0.2 ml蒸馏水灌胃,各组共灌胃3个月。比较各组小鼠发情周期、产仔数量,体外受精的获卵数、成熟卵子数、受精卵数、卵裂数及囊胚数,检测卵巢中新生儿卵巢同源盒基因(Nobox)蛋白及mRNA表达情况。结果与青年组比较,模型组小鼠完整发情周期次数、发情前期及发情期占周期比例及产仔数减少,获卵数、成熟卵子数、受精卵数、卵裂数及囊胚数亦减少,卵巢中Nobox蛋白及mRNA表达降低(P<0.05或P<0.01);补肾健脾方高剂量组小鼠在体外受精过程中获卵数、成熟卵子数、受精卵数、卵裂数、囊胚数均高于模型组,并且卵巢中Nobox蛋白及mRNA表达升高(P<0.05或P<0.01)。结论补肾健脾方有助于改善自然衰老小鼠生殖能力及卵子质量,其作用机制可能与增加小鼠卵巢内Nobox转录因子表达有关。Objective To observe the effect of Bushen Jianpi Formula（ 补肾健脾方） on ovarian function in natural aging mice and its possible mechanism. Methods Randomly divided 48 C57 BL/6 J female mice of 9 months old（ natural aging models） into model group,positive control group,Bushen Jianpi Formula high and low dose group,with 12 mice in each group. Other 12 mice aged 2 ~ 3 months were selected as the youth group. The Bushen Jianpi Formula low and high dose group were given Bushen Jianpi Formula 0. 51 and 2. 55 g/（ kg · d） by gavage respectively,while the positive control group were given Dehydroepiandrosterone tablet 1. 25 mg（ kg·d） by gavage,and the youth group and model group were given 0. 2 ml distilled water by gavage,all for 3 months. Compared estrous cycle,farrowing amount,numbers of retrieved oocytes in vitro fertilization,mature oocytes,fertilized eggs,cleavage number and number of blastocyst and measured expression of newborn ovary homeobox gene（ Nobox） protein and mRNA in ovary of mice in different groups. Results Compared with the youth group,the whole estrous cycles times,proportion of proestrus and oestrum in whole cycle,farrowing amount decreased in the model group. The number of retrieved oocytes in vitro fertilization,mature oocytes,fertilized eggs,cleavage number and number of blastocyst were also reduced with reduction of the expression of Nobox protein and mRNA in ovary（ P〈 0. 05 or P 〈0. 01）.Meanwhile,the number of retrieved oocytes in vitro fertilization,mature oocytes,fertilized eggs,cleavage number and number of blastocyst in the Bushen Jianpi Formula high dose group were higher than that in the model group,and the expression of Nobox protein and mRNA in the ovary increased（ P 〈0. 05 or P〈 0. 01）. Conclusion Bushen Jianpi Formula could improve the reproductive ability and egg quality of natural aging mice,and its mechanism may be related to increasing Nobox transcription factor expression in the ovary of mice.

关 键 词：自然衰老 补肾健脾方 生殖能力 体外受精 卵子质量 新生儿卵巢同源盒基因 

分 类 号：R285.5[医药卫生—中药学]