白念珠菌Mdr1基因生物信息学分析  

The bioinformatical analysis of Mdr1 gene from Candida albicans

作　　者：何泰龙 高文超[1] 孙瑶[1] 黄家敏[1] 黄丽林[2] 张静[1,3] 张云青[1] 李美荣[1] 尹颂超[1] 黄怀球[1] 

机构地区：[1]中山大学附属第三医院皮肤科,广东广州510630 [2]广东省妇幼保健院儿科,广东广州510010 [3]中山大学孙逸仙纪念医院皮肤科,广东广州510120

出　　处：《皮肤性病诊疗学杂志》2017年第5期307-311,共5页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：国家自然科学基金面上项目(编号:81371746);广东省科技计划项目(编号:2013B010404013)

摘　　要：目的:探讨白念珠菌多重耐药基因(Mdr1)DNA、mRNA、蛋白的潜在生物学性质。方法:采用生物信息学方法对白念珠菌Mdr1基因DNA、mRNA、蛋白序列进行分析。结果:Mdr1 DNA中无内含子,Mdr1 mRNA中含有多个调控元件和腺苷酸化位点、AU富集区,对二级结构分析显示,Mdr1 mRNA全长序列可形成棒状结构。蛋白质的理化性质分析显示MDR1蛋白稳定系数为37.14,提示MDR1蛋白较为稳定。Target P 1.1和Signal P 4.1预测结果显示MDR1蛋白不含有线粒体定位序列和分泌信号肽。SWISS-MODEL 3D建模,MDR1蛋白质的3D结构类似于通道管状形态。Motif Scan分析结果显示,MDR1蛋白第116-557个氨基酸构成主要协同转运蛋白超家族,TMHMM预测在此范围内含有12个跨膜区间,为跨膜蛋白。结论:Mdr1基因的表达可能受其它基因的调控,MDR1蛋白为主要协同转运蛋白超家族成员,其功能域或为潜在的药物靶点。Objective：To explore the biological characters of the DNA, mRNA and protein se- quence of Mdrl gene from Candida albicans. Methods ： The bioinformatical analysis of DNA, mR- NA and protein sequence of Mdrl gene from Candida albicans was conducted. Results： Mdrl gene had no introns. Mdrl mRNA have a few multiple regulatory element, polyadenylation site and AU enrichment region. RNA structure analysis showed that Mdrl mRNA form is a rodlike. The analysis of the physical and chemical properties showed that the stabilizing factor of MDR1 protein was 37.14. TargetP 1.1 and SignalP 4.1 predicted that Mdrl gene was lack of mitochon- drial localization sequence and secretion signal peptide. The 3D structure of MDR1 protein is simi- lar to the channel tubular which morphology was draw by SWISS-MODEL. Motif analysis revealed that the major facilitator superfamily protein was consist of the 116-557 rst amino acids of MDR1,and TMHMM analysis indicated that 12 transmembrane domains were included in the region. Con- clusion： The expression of Mdrl gene may be regulated by other genes. MDR1 protein is one of the major members of major facilitator superfamily,which might be a potential target

关 键 词：白念珠菌 MDR1基因 生物信息学 

分 类 号：R379.4[医药卫生—病原生物学]