Plant regeneration from cultured protoplasts of a glutinous rice  

作　　者：Wang Guangyuan ,Hsia Chenau(Xia Zhenao), Chi Jinfen and Gao XiaoyanShanghai Institute of Plant Physiology, Academia Sinica 

出　　处：《Cell Research》1990年第1期17-21,共5页细胞研究（英文版）

摘　　要：Young embryos of rice (Oryza saliva L. subsp. japonica var. Guo-xiang No.l) were cultured on MS agar medium(2,4-D 2 mg/l). Calli were formed and subcultured on N6 agar medium (2,4-D 2 mg/l). After selection, the small, grainy and pale yellowish cell clusters with dense cytoplasm were used in protoplast preparation. Isolated protoplasts were cultured in N6 medium (2,4-D 1 mg/l, 6-BA 0.2 mg/l)1 with agarose block culture method. The protoplasts grew, divided and formed calli. After inducing differentiation, the regenerated mature plants were obtained.

关 键 词：protoplast culture Oryza saliva glutinous va-riety plant regeneration. 

分 类 号：S511[农业科学—作物学] Q943.1[生物学—植物学]