In vitro Induction of primary antibody responses to particulate and soluble protein antigens in T cell-replaced murine spleen cell cultures  

作　　者：Lu KunShanghai Institute of Cell Biology, Academia Sinica,Shanghai,200031,China. 

出　　处：《Cell Research》1990年第1期23-33,共11页细胞研究（英文版）

摘　　要：Specific antibody responses could be induced in serumfree condition.Specific anti-SRBC or anti-SRBC ghost antibody were induced from anti-Thy treated (T-depleted) murine spleen cells in serum-free culture in the presence of Con A conditioned medium.This induction system may facilitate the study of lymphokine functions on antigen triggered B cells. In T cell-replaced cultures,the antibody responses of B cells could be successfully induced when soluble SRBC membrane proteins were used as antigens.It thus indicates that antigen together with lymphokines are sufficient to drive B cells to become antibody secreting cells in the absence of T cells.The T cell-replaced system provides a more stable way for in vitro immunization and may be applied to monoclonal antibody production when in vivo immunization is difficult to be carried out.

关 键 词：B cell maturation LYMPHOKINES in vitro immunization. 

分 类 号：R392.12[医药卫生—免疫学]