FXR通过Akt信号通路调节人肝癌细胞SMMC-7721的细胞增殖  

Farnesoid X receptor regulates the proliferation of human hepatoma cell line SMMC-7721 via the Akt signaling pathway

作　　者：张春燕 陶忠桦 陈川宁 甘淋 

机构地区：[1]西南医科大学生物化学与分子生物学教研室,四川泸州646000 [2]西南医科大学公共卫生学院实验教学中心,四川泸州646000

出　　处：《西南医科大学学报》2017年第5期449-451,462,共4页Journal of Southwest Medical University

基　　金：国家自然基金项目(81341121);四川省教育厅重点项目(15ZA0161)

摘　　要：目的:探讨法尼醇X受体(FXR)表达对人肝癌细胞SMMC-7721细胞增殖的影响及分子机制。方法:在人正常肝细胞株L02、肝癌细胞株Hep3B和SMMC-7721中检测FXR的表达;分别采用FXR激动剂GW4064和抑制剂INT747干预FXR的表达,通过CCK-8实验检测FXR表达改变对SMMC-7721细胞株细胞增殖的影响;进一步用WB检测参与FXR表达改变对Akt信号通路的影响。结果:WB结果显示较L02,FXR在Hep3B和SMMC-7721表达降低;CCK-8结果提示在SMMC-7721细胞中,FXR表达增高降低细胞增殖(P<0.05),反之则促进细胞的生长和活性(P<0.05);WB结果显示FXR表达改变能负调节Akt的表达。结论:FXR可能通过Akt信号通路调节人肝癌细胞SMMC-7721的细胞增殖。Objective： To investigate the effect of farnesoid X receptor （FXR） expression on the proliferation of human hepatoma cell line SMMC-7721 and related molecular mechanism. Methods： The expression of FXR was measuredin the normal humanliver cells L02 and hepatoma cell lines Hep3B and SMMC-7721. An FXR agonist GW4064 and an FXR inhibitor INT747 were used to interfere with the expression of FXR. Cell Counting Kit-8 （CCK-8）was used to investigate the effect of the change in FRX expression on the proliferation of SMMC- 7721 cells, and Western blot was used to analyze the effect of the change in FRX expression on the Akt signaling pathway. Results： The results of Western blot showed that Hep3B and SMMC-7721 cells had lower FXR expression than L02 cells.CCK-8 showed thatincreased FXR expression significantly reduced the proliferation of SMMC-7721 cells （P 〈 0.05）, while a reduction in FXR expression significantly increased the growth and viability of cells. Western blot also showed that the change in FXR expression negatively regulated the expression of Akt. Conclusions： FXR regulates theproliferation ofhuman hepatoma SMMC-7721cellsvia the Akt signaling pathway.

关 键 词：FXK 肝癌 细胞增殖 AKT 

分 类 号：R614.2[医药卫生—麻醉学]