外加碳源对红球菌IcdP1降解荧蒽的特性研究  

Effects of External Carbon Sources on Degradation of Fluoranthene by Rhodococcus sp. Icd P1

作　　者：夏瑛铭 张朝晖[1,2] 王亮[1,2] 赵斌[1,2] 左宇环 张敏[1] 李君敬[1,2] 惠旭[1] 

机构地区：[1]天津工业大学环境与化学工程学院 [2]天津工业大学省部共建分离膜与膜过程国家重点实验室,天津300387

出　　处：《环境科学与技术》2017年第9期14-19,共6页Environmental Science & Technology

基　　金：国家自然科学基金资助项目(51678408;51638011)

摘　　要：从受多环芳烃(PAHs)污染的河道底泥中筛选分离、纯化得到一株荧蒽降解菌,经形貌分析和16S r DNA序列分析后确定该菌株为红球菌属Rhodococcus sp.,命名为Icd P1。之后对该菌株降解荧蒽性能进行了系统研究。结果表明,当荧蒽浓度为50 mg/L时,Icd P1在培养42 d后对荧蒽降解率可到达31.69%。投加"蒽-菲"混合物、葡萄糖、麦芽糖、腐殖酸(HA)等外加碳源可以不同程度强化Icd P1降解荧蒽的能力,其中投加葡萄糖对红球菌降解荧蒽的强化效果最好,且荧蒽去除率和红球菌生物量之间的线性相关关系最好,说明葡萄糖最适合作为该红球菌降解荧蒽的共代谢基质。动力学分析表明,无论是否添加葡萄糖作为外加碳源,红球菌Icd P1对荧蒽的降解都符合一级动力学降解模型,但葡萄糖的加入大大提高了Icd P1菌对荧蒽的降解速率。A fluoranthene-degrading bacterial strain named as Icd P1 was isolated from the river sediment which has been contaminated by polycyclic aromatic hydrocarbons（PAHs）. This strain was identified as Rhodococcus sp. through morphological observation and 16 S r DNA sequences homology analysis. The degradation properties of fluoranthene by the strain were studied. The results showed that when the initial concentration of fluoranthene was 50 mg/L, 31.69 % of the total was degraded by Icd P1 after 42 d. The degradation ability of Icd P1 for fluoranthene could be enhanced after adding external carbon sources such as mixture of anthracene phenanthrene, glucose, maltose and humic acid（HA）. Among these external carbon sources, the most obvious reinforcement effect was achieved for glucose, and the best linear correlation relationship between biomass and fluoranthene removal was also obtained. The result suggested that glucose was suitable for fluoranthene cometabolism as biodegradation substrate of Icd P1. Dynamics analysis showed that the degradation follows the first order kinetic degradation model whether or not to add glucose as external carbon source. However, the removal efficiency of fluoranthene by Icd P1 was markedly increased after adding glucose.

关 键 词：荧蒽 红球菌 外加碳源 河道底泥 降解 

分 类 号：X506[环境科学与工程—环境工程]