HIV-1 can infect northern pig-tailed macaques(Macaca leonina) and form viral reservoirs in vivo  

作　　者：Wei Pang Gao-Hong Zhang Jin Jiang Hong-Yi Zheng Lin-Tao Zhang Xiao-Liang Zhang Jia-Hao Song Ming-Xu Zhang Jia-Wu Zhu Ai-Hua Lei Ren-Rong Tian Xiao-Ming Liu Liguo Zhang Guangxia Gao Lishan Su Yong-Tang Zheng 

机构地区：[1]Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province,Kunming Institute of Zoology,Chinese Academy of Sciences [2]University of Chinese Academy of Sciences [3]Institute of Health Sciences,Anhui University [4]South China Institute of Endangered Animal,Guandong Academy of Sciences [5]Key Laboratory of Infection and Immunity,Institute of Biophysics,Chinese Academy of Sciences [6]Department of Microbiology and Immunology,School of Medicine,The University of North Carolina at Chapel Hill

出　　处：《Science Bulletin》2017年第19期1315-1324,共10页科学通报（英文版）

基　　金：supported by the National Basic Research Program of China (2012CBA01305);the National Natural Science Foundation of China (81172876; 81571606; 81471620; U0832601; 30671960);the 13th Five-Year Key Scientific and Technological Program of China (2017ZX10304402002-004; 2017ZX10202102-001-005);the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-R-13; KJZD-EW-L10-02; CASIMM0320163020);the National Key Research & Development Plan (2016YFC1201000);Yunnan Applicative and Basic Research Program (2014FB181; 201501PH00015)

摘　　要：Viral reservoirs of HIV-1 are a major obstacle for curing AIDS. The novel animal models that can be directly infected with HIV-I will contribute to develop effective strategies for eradicating infections. Here, we inoculated 4 northern pig-tailed macaques （NPM） with the HIV-1 strain HIV-1NL4.3 and moni- tored the infection for approximately 3 years （150 weeks）. The HIV-l-infected NPMs showed transient viremia for about 10 weeks after infection. However, cell-associated proviral DNA and viral RNA persisted in the peripheral blood and lymphoid organs for about 3 years. Moreover, replication-competent HIV-1 could be successfully recovered from peripheral blood mononuclear cells （PBMCs） during long-term infection. The numbers of resting CD4~ T cells in HIV-1 infected NPMs harboring proviruses fell within a range of 2- to 3-1oglo per million cells, and these proviruses could be reactivated both ex vivo and in vivo in response to co-stimulation with the latency-reversing agents JQ1 and prostratin. Our results suggested that NPMs can be infected with HIV-1 and a long-term viral reservoir was formed in NPMs, which might serve as a potential model for HIV-1 reservoir research.Viral reservoirs of HIV-1 are a major obstacle for curing AIDS. The novel animal models that can be directly infected with HIV-1 will contribute to develop effective strategies for eradicating infections.Here, we inoculated 4 northern pig-tailed macaques(NPM) with the HIV-1 strain HIV-1_(NL4.3) and monitored the infection for approximately 3 years(150 weeks). The HIV-1-infected NPMs showed transient viremia for about 10 weeks after infection. However, cell-associated proviral DNA and viral RNA persisted in the peripheral blood and lymphoid organs for about 3 years. Moreover, replication-competent HIV-1could be successfully recovered from peripheral blood mononuclear cells(PBMCs) during long-term infection. The numbers of resting CD4^+T cells in HIV-1 infected NPMs harboring proviruses fell within a range of 2-to 3-log_(10) per million cells, and these proviruses could be reactivated both ex vivo and in vivo in response to co-stimulation with the latency-reversing agents JQ1 and prostratin. Our results suggested that NPMs can be infected with HIV-1 and a long-term viral reservoir was formed in NPMs,which might serve as a potential model for HIV-1 reservoir research.

关 键 词：Northern pig-tailed macaquesHIV-1 Viral reservoir JQ1 Prostratin 

分 类 号：R512.91[医药卫生—内科学]